In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus)

Takashi Todo, Mana Sato, Misa Ashida, Naohiro Yamaguchi, Yasuhisa Kobayashi, Shinji Adachi, Kohei Yamauchi, Masaru Nakamura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recently, we succeeded in inducing in vitro spermatogenesis in a protogynous hermaphrodite fish, threespotted wrasse (Halichoeres trimaculatus), during organ culture of ovary with a serum-supplemented medium. In the present study, we further optimized and used this culture system to determine effective steroid doses to induce sex change, and to investigate the importance of apoptosis during sex change in vitro. The results show that the restructuring from ovary to testis in the wrasse could be induced in vitro with a serum-free medium. Gonadal sex change can be triggered during basic in vitro culture due to a lack of endogenous factors (especially, estrogens) for female sexuality, and can be accelerated by androgens. Furthermore, oocyte apoptosis may be an important mechanism effecting gonadal sex change.

Original languageEnglish
Pages (from-to)106
Number of pages1
JournalCybium
Volume32
Issue number2 SUPPL.
Publication statusPublished - Jul 31 2008
Externally publishedYes

Fingerprint

Labridae
sex reversal
apoptosis
serum
hermaphrodite
sexuality
androgen
steroid
fish
organ culture
spermatogenesis
androgens
in vitro culture
estrogens
steroids
testes
oocytes
Halichoeres
dosage
dose

Keywords

  • Organ culture
  • Ovary
  • Protogynous fish
  • Sex change
  • Spermatogenesis
  • Wrasse

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

Cite this

Todo, T., Sato, M., Ashida, M., Yamaguchi, N., Kobayashi, Y., Adachi, S., ... Nakamura, M. (2008). In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus). Cybium, 32(2 SUPPL.), 106.

In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus). / Todo, Takashi; Sato, Mana; Ashida, Misa; Yamaguchi, Naohiro; Kobayashi, Yasuhisa; Adachi, Shinji; Yamauchi, Kohei; Nakamura, Masaru.

In: Cybium, Vol. 32, No. 2 SUPPL., 31.07.2008, p. 106.

Research output: Contribution to journalArticle

Todo, T, Sato, M, Ashida, M, Yamaguchi, N, Kobayashi, Y, Adachi, S, Yamauchi, K & Nakamura, M 2008, 'In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus)', Cybium, vol. 32, no. 2 SUPPL., pp. 106.
Todo T, Sato M, Ashida M, Yamaguchi N, Kobayashi Y, Adachi S et al. In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus). Cybium. 2008 Jul 31;32(2 SUPPL.):106.
Todo, Takashi ; Sato, Mana ; Ashida, Misa ; Yamaguchi, Naohiro ; Kobayashi, Yasuhisa ; Adachi, Shinji ; Yamauchi, Kohei ; Nakamura, Masaru. / In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus). In: Cybium. 2008 ; Vol. 32, No. 2 SUPPL. pp. 106.
@article{54d4e18c95cf43fab92bc74c9d5a8924,
title = "In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus)",
abstract = "Recently, we succeeded in inducing in vitro spermatogenesis in a protogynous hermaphrodite fish, threespotted wrasse (Halichoeres trimaculatus), during organ culture of ovary with a serum-supplemented medium. In the present study, we further optimized and used this culture system to determine effective steroid doses to induce sex change, and to investigate the importance of apoptosis during sex change in vitro. The results show that the restructuring from ovary to testis in the wrasse could be induced in vitro with a serum-free medium. Gonadal sex change can be triggered during basic in vitro culture due to a lack of endogenous factors (especially, estrogens) for female sexuality, and can be accelerated by androgens. Furthermore, oocyte apoptosis may be an important mechanism effecting gonadal sex change.",
keywords = "Organ culture, Ovary, Protogynous fish, Sex change, Spermatogenesis, Wrasse",
author = "Takashi Todo and Mana Sato and Misa Ashida and Naohiro Yamaguchi and Yasuhisa Kobayashi and Shinji Adachi and Kohei Yamauchi and Masaru Nakamura",
year = "2008",
month = "7",
day = "31",
language = "English",
volume = "32",
pages = "106",
journal = "Cybium",
issn = "0399-0974",
publisher = "Societe Francaise d'Ichtyologie",
number = "2 SUPPL.",

}

TY - JOUR

T1 - In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus)

AU - Todo, Takashi

AU - Sato, Mana

AU - Ashida, Misa

AU - Yamaguchi, Naohiro

AU - Kobayashi, Yasuhisa

AU - Adachi, Shinji

AU - Yamauchi, Kohei

AU - Nakamura, Masaru

PY - 2008/7/31

Y1 - 2008/7/31

N2 - Recently, we succeeded in inducing in vitro spermatogenesis in a protogynous hermaphrodite fish, threespotted wrasse (Halichoeres trimaculatus), during organ culture of ovary with a serum-supplemented medium. In the present study, we further optimized and used this culture system to determine effective steroid doses to induce sex change, and to investigate the importance of apoptosis during sex change in vitro. The results show that the restructuring from ovary to testis in the wrasse could be induced in vitro with a serum-free medium. Gonadal sex change can be triggered during basic in vitro culture due to a lack of endogenous factors (especially, estrogens) for female sexuality, and can be accelerated by androgens. Furthermore, oocyte apoptosis may be an important mechanism effecting gonadal sex change.

AB - Recently, we succeeded in inducing in vitro spermatogenesis in a protogynous hermaphrodite fish, threespotted wrasse (Halichoeres trimaculatus), during organ culture of ovary with a serum-supplemented medium. In the present study, we further optimized and used this culture system to determine effective steroid doses to induce sex change, and to investigate the importance of apoptosis during sex change in vitro. The results show that the restructuring from ovary to testis in the wrasse could be induced in vitro with a serum-free medium. Gonadal sex change can be triggered during basic in vitro culture due to a lack of endogenous factors (especially, estrogens) for female sexuality, and can be accelerated by androgens. Furthermore, oocyte apoptosis may be an important mechanism effecting gonadal sex change.

KW - Organ culture

KW - Ovary

KW - Protogynous fish

KW - Sex change

KW - Spermatogenesis

KW - Wrasse

UR - http://www.scopus.com/inward/record.url?scp=73349127729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73349127729&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:73349127729

VL - 32

SP - 106

JO - Cybium

JF - Cybium

SN - 0399-0974

IS - 2 SUPPL.

ER -