In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus)

Takashi Todo, Mana Sato, Misa Ashida, Naohiro Yamaguchi, Yasuhisa Kobayashi, Shinji Adachi, Kohei Yamauchi, Masaru Nakamura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Recently, we succeeded in inducing in vitro spermatogenesis in a protogynous hermaphrodite fish, threespotted wrasse (Halichoeres trimaculatus), during organ culture of ovary with a serum-supplemented medium. In the present study, we further optimized and used this culture system to determine effective steroid doses to induce sex change, and to investigate the importance of apoptosis during sex change in vitro. The results show that the restructuring from ovary to testis in the wrasse could be induced in vitro with a serum-free medium. Gonadal sex change can be triggered during basic in vitro culture due to a lack of endogenous factors (especially, estrogens) for female sexuality, and can be accelerated by androgens. Furthermore, oocyte apoptosis may be an important mechanism effecting gonadal sex change.

Original languageEnglish
Number of pages1
JournalCybium
Volume32
Issue number2 SUPPL.
Publication statusPublished - Jul 31 2008

Keywords

  • Organ culture
  • Ovary
  • Protogynous fish
  • Sex change
  • Spermatogenesis
  • Wrasse

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

Fingerprint Dive into the research topics of 'In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus)'. Together they form a unique fingerprint.

  • Cite this

    Todo, T., Sato, M., Ashida, M., Yamaguchi, N., Kobayashi, Y., Adachi, S., Yamauchi, K., & Nakamura, M. (2008). In vitro induction of gonadal sex change in a protogynous fish, three-spotted wrasse (Halichoeres trimaculatus). Cybium, 32(2 SUPPL.).