In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology

Yoshitsugu Matsumoto, Masahiro Kuroda, Ryohei Matsuya, Hirokazu Kato, Koichi Shibuya, Masataka Oita, Atsushi Kawabe, Hidenobu Matsuzaki, Jun-Ichi Asaumi, Jun Murakami, Kazunori Katashima, Masakazu Ashida, Takanori Sasaki, Tetsuro Sei, Susumu Kanazawa, Seiichi Mimura, Seiichiro Oono, Takuichi Kitayama, Seiji Tahara, Keiji Inamura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Diffusion-weighted magnetic resonance imaging (MRI) is frequently used clinically, and is available for the whole-body screening for tumors. The exact mechanism by which the apparent diffusion coefficient (ADC) value decreases in tumorous tissue remains unclear, although various theories have been proposed, including intracellular and extracellular factor theories. It is impossible to distinguish each factor in the intracellular and extracellular spaces as the source of MR signal generation by means of conventional comparison between MR images and pathological specimens. Other factors which have been reported to affect ADC include cellularity and cellular edema of human tissues, and temperature of phantoms at the time of measurement. We employed a new technique that enables cellular MR imaging using a newly developed bio-phantom containing a living culture tumor cell line, Jurkat-N1. We investigated possible reasons for observed decreases in ADC values for tumors, and we considered the contribution of both the intracellular and extracellular space to such a decrease. The ADC values of the bio-phantom increased with increasing heat exposure from 27 to 45°C. ADC values also increased after the destruction by sonication of tumor cell membranes. ADC values decreased as cellularity increased in the bio-phantom. ADC values decreased due to cellular edema caused by a low salt concentration in the bio-phantom. Changes in pressure in the bio-phantom had no effect on the observed ADC values. We calculated both the intracellular ADC and extracellular ADC values using the ADC values, cellularity, and cellular volume of Jurkat-N1 cells in the bio-phantom. The extracellular ADC values in the bio-phantom were estimated to be lower than the ADC value of distilled water. These results indicate that not only intracellular ADC values, but also extracellular ADC values contribute to the determination of the ADC values of bio-phantoms. This is the first report to have examined the contribution of intracellular and extracellular space on the ADC values of bio-phantoms containing cultured tumor cells.

Original languageEnglish
Pages (from-to)641-648
Number of pages8
JournalOncology Reports
Volume22
Issue number3
DOIs
Publication statusPublished - 2009

Fingerprint

Intracellular Space
Extracellular Space
In Vitro Techniques
Edema
Cultured Tumor Cells
Whole Body Imaging
Neoplasms
Sonication
Diffusion Magnetic Resonance Imaging
Jurkat Cells
Tumor Cell Line
Salts
Hot Temperature
Cell Membrane
Pressure
Temperature
Water

Keywords

  • ADC value
  • Diffusion-weighted image
  • Extracellular space
  • Intracellular space
  • MRI
  • Phantom

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology. / Matsumoto, Yoshitsugu; Kuroda, Masahiro; Matsuya, Ryohei; Kato, Hirokazu; Shibuya, Koichi; Oita, Masataka; Kawabe, Atsushi; Matsuzaki, Hidenobu; Asaumi, Jun-Ichi; Murakami, Jun; Katashima, Kazunori; Ashida, Masakazu; Sasaki, Takanori; Sei, Tetsuro; Kanazawa, Susumu; Mimura, Seiichi; Oono, Seiichiro; Kitayama, Takuichi; Tahara, Seiji; Inamura, Keiji.

In: Oncology Reports, Vol. 22, No. 3, 2009, p. 641-648.

Research output: Contribution to journalArticle

Matsumoto, Y, Kuroda, M, Matsuya, R, Kato, H, Shibuya, K, Oita, M, Kawabe, A, Matsuzaki, H, Asaumi, J-I, Murakami, J, Katashima, K, Ashida, M, Sasaki, T, Sei, T, Kanazawa, S, Mimura, S, Oono, S, Kitayama, T, Tahara, S & Inamura, K 2009, 'In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology', Oncology Reports, vol. 22, no. 3, pp. 641-648. https://doi.org/10.3892/or_00000484
Matsumoto, Yoshitsugu ; Kuroda, Masahiro ; Matsuya, Ryohei ; Kato, Hirokazu ; Shibuya, Koichi ; Oita, Masataka ; Kawabe, Atsushi ; Matsuzaki, Hidenobu ; Asaumi, Jun-Ichi ; Murakami, Jun ; Katashima, Kazunori ; Ashida, Masakazu ; Sasaki, Takanori ; Sei, Tetsuro ; Kanazawa, Susumu ; Mimura, Seiichi ; Oono, Seiichiro ; Kitayama, Takuichi ; Tahara, Seiji ; Inamura, Keiji. / In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology. In: Oncology Reports. 2009 ; Vol. 22, No. 3. pp. 641-648.
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AU - Shibuya, Koichi

AU - Oita, Masataka

AU - Kawabe, Atsushi

AU - Matsuzaki, Hidenobu

AU - Asaumi, Jun-Ichi

AU - Murakami, Jun

AU - Katashima, Kazunori

AU - Ashida, Masakazu

AU - Sasaki, Takanori

AU - Sei, Tetsuro

AU - Kanazawa, Susumu

AU - Mimura, Seiichi

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AU - Tahara, Seiji

AU - Inamura, Keiji

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