In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells

Yusuke Nakashima, Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp powders were synthesized with desired Si contents (0, 0.4, 0.8, 1.6, and 2.4 mass%) as a nominal composition. Another previous study in our group demonstrated surface-modification of HAp powder with inositol phosphate (IP6) enhanced the compressive strength of apatite cement. Thus, to fabricate the cements with higher bioactivity, the above Si-HAp powders were surface-modified with IP6 (IP6-Si-HAp). The IP6-Si-HAp cements with various Si contents were fabricated by mixing with pure water at the powder/liquid ratio of 1/0.4 [w/v]. In order to clarify biocompatibility of the IP6-Si-HAP cements in the present work, MC3T3-E1 cells as a model of osteoblast were seeded on the cement specimens. As for the numbers of cells cultured on the IP6-Si-HAp cements, the substitution of lower levels of Si into HAp lattice did not greatly influence the cell proliferation. However, the substitution of Si amount over 0.8 mass% enhanced the cell proliferation. Especially, the IP6-Si-HAp cement with the Si content of 2.4 mass% showed excellent cell proliferation among examined specimens. Therefore, to fabricate the cements with higher bioactivity, it is necessary to control the amount of Si in the IP6-Si-HAp cements. The usage of these IP6-Si-HAp cements may make it possible to fabricate the cements with higher bioactivity, compare to conventional pure HAp cements.

Original languageEnglish
Title of host publicationKey Engineering Materials
Pages183-186
Number of pages4
Volume529-530
Edition1
DOIs
Publication statusPublished - 2013
Externally publishedYes
Event24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan
Duration: Oct 21 2012Oct 24 2012

Publication series

NameKey Engineering Materials
Number1
Volume529-530
ISSN (Print)10139826

Other

Other24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
CountryJapan
CityFukuoka
Period10/21/1210/24/12

Fingerprint

Apatites
Apatite
Silicon
Hydroxyapatite
Bone cement
Powders
Cements
Durapatite
Cell proliferation
Bioactivity
Substitution reactions
Inositol Phosphates
Osteoblasts
Biocompatibility
Compressive strength
Surface treatment
Phosphates
hydroxyapatite cement
Water

Keywords

  • Apatite
  • Cement
  • Osteoblast
  • Silicon
  • Silicon-containing hydroxyapatite

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Nakashima, Y., Honda, M., Konishi, T., Mizumoto, M., & Aizawa, M. (2013). In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells. In Key Engineering Materials (1 ed., Vol. 529-530, pp. 183-186). (Key Engineering Materials; Vol. 529-530, No. 1). https://doi.org/10.4028/www.scientific.net/KEM.529-530.183

In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells. / Nakashima, Yusuke; Honda, Michiyo; Konishi, Toshiisa; Mizumoto, Minori; Aizawa, Mamoru.

Key Engineering Materials. Vol. 529-530 1. ed. 2013. p. 183-186 (Key Engineering Materials; Vol. 529-530, No. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakashima, Y, Honda, M, Konishi, T, Mizumoto, M & Aizawa, M 2013, In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells. in Key Engineering Materials. 1 edn, vol. 529-530, Key Engineering Materials, no. 1, vol. 529-530, pp. 183-186, 24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012, Fukuoka, Japan, 10/21/12. https://doi.org/10.4028/www.scientific.net/KEM.529-530.183
Nakashima Y, Honda M, Konishi T, Mizumoto M, Aizawa M. In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells. In Key Engineering Materials. 1 ed. Vol. 529-530. 2013. p. 183-186. (Key Engineering Materials; 1). https://doi.org/10.4028/www.scientific.net/KEM.529-530.183
Nakashima, Yusuke ; Honda, Michiyo ; Konishi, Toshiisa ; Mizumoto, Minori ; Aizawa, Mamoru. / In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells. Key Engineering Materials. Vol. 529-530 1. ed. 2013. pp. 183-186 (Key Engineering Materials; 1).
@inproceedings{dc9b6dd09e2c494caf6303209c119874,
title = "In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells",
abstract = "In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp powders were synthesized with desired Si contents (0, 0.4, 0.8, 1.6, and 2.4 mass{\%}) as a nominal composition. Another previous study in our group demonstrated surface-modification of HAp powder with inositol phosphate (IP6) enhanced the compressive strength of apatite cement. Thus, to fabricate the cements with higher bioactivity, the above Si-HAp powders were surface-modified with IP6 (IP6-Si-HAp). The IP6-Si-HAp cements with various Si contents were fabricated by mixing with pure water at the powder/liquid ratio of 1/0.4 [w/v]. In order to clarify biocompatibility of the IP6-Si-HAP cements in the present work, MC3T3-E1 cells as a model of osteoblast were seeded on the cement specimens. As for the numbers of cells cultured on the IP6-Si-HAp cements, the substitution of lower levels of Si into HAp lattice did not greatly influence the cell proliferation. However, the substitution of Si amount over 0.8 mass{\%} enhanced the cell proliferation. Especially, the IP6-Si-HAp cement with the Si content of 2.4 mass{\%} showed excellent cell proliferation among examined specimens. Therefore, to fabricate the cements with higher bioactivity, it is necessary to control the amount of Si in the IP6-Si-HAp cements. The usage of these IP6-Si-HAp cements may make it possible to fabricate the cements with higher bioactivity, compare to conventional pure HAp cements.",
keywords = "Apatite, Cement, Osteoblast, Silicon, Silicon-containing hydroxyapatite",
author = "Yusuke Nakashima and Michiyo Honda and Toshiisa Konishi and Minori Mizumoto and Mamoru Aizawa",
year = "2013",
doi = "10.4028/www.scientific.net/KEM.529-530.183",
language = "English",
isbn = "9783037855171",
volume = "529-530",
series = "Key Engineering Materials",
number = "1",
pages = "183--186",
booktitle = "Key Engineering Materials",
edition = "1",

}

TY - GEN

T1 - In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells

AU - Nakashima, Yusuke

AU - Honda, Michiyo

AU - Konishi, Toshiisa

AU - Mizumoto, Minori

AU - Aizawa, Mamoru

PY - 2013

Y1 - 2013

N2 - In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp powders were synthesized with desired Si contents (0, 0.4, 0.8, 1.6, and 2.4 mass%) as a nominal composition. Another previous study in our group demonstrated surface-modification of HAp powder with inositol phosphate (IP6) enhanced the compressive strength of apatite cement. Thus, to fabricate the cements with higher bioactivity, the above Si-HAp powders were surface-modified with IP6 (IP6-Si-HAp). The IP6-Si-HAp cements with various Si contents were fabricated by mixing with pure water at the powder/liquid ratio of 1/0.4 [w/v]. In order to clarify biocompatibility of the IP6-Si-HAP cements in the present work, MC3T3-E1 cells as a model of osteoblast were seeded on the cement specimens. As for the numbers of cells cultured on the IP6-Si-HAp cements, the substitution of lower levels of Si into HAp lattice did not greatly influence the cell proliferation. However, the substitution of Si amount over 0.8 mass% enhanced the cell proliferation. Especially, the IP6-Si-HAp cement with the Si content of 2.4 mass% showed excellent cell proliferation among examined specimens. Therefore, to fabricate the cements with higher bioactivity, it is necessary to control the amount of Si in the IP6-Si-HAp cements. The usage of these IP6-Si-HAp cements may make it possible to fabricate the cements with higher bioactivity, compare to conventional pure HAp cements.

AB - In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp powders were synthesized with desired Si contents (0, 0.4, 0.8, 1.6, and 2.4 mass%) as a nominal composition. Another previous study in our group demonstrated surface-modification of HAp powder with inositol phosphate (IP6) enhanced the compressive strength of apatite cement. Thus, to fabricate the cements with higher bioactivity, the above Si-HAp powders were surface-modified with IP6 (IP6-Si-HAp). The IP6-Si-HAp cements with various Si contents were fabricated by mixing with pure water at the powder/liquid ratio of 1/0.4 [w/v]. In order to clarify biocompatibility of the IP6-Si-HAP cements in the present work, MC3T3-E1 cells as a model of osteoblast were seeded on the cement specimens. As for the numbers of cells cultured on the IP6-Si-HAp cements, the substitution of lower levels of Si into HAp lattice did not greatly influence the cell proliferation. However, the substitution of Si amount over 0.8 mass% enhanced the cell proliferation. Especially, the IP6-Si-HAp cement with the Si content of 2.4 mass% showed excellent cell proliferation among examined specimens. Therefore, to fabricate the cements with higher bioactivity, it is necessary to control the amount of Si in the IP6-Si-HAp cements. The usage of these IP6-Si-HAp cements may make it possible to fabricate the cements with higher bioactivity, compare to conventional pure HAp cements.

KW - Apatite

KW - Cement

KW - Osteoblast

KW - Silicon

KW - Silicon-containing hydroxyapatite

UR - http://www.scopus.com/inward/record.url?scp=84871285954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871285954&partnerID=8YFLogxK

U2 - 10.4028/www.scientific.net/KEM.529-530.183

DO - 10.4028/www.scientific.net/KEM.529-530.183

M3 - Conference contribution

AN - SCOPUS:84871285954

SN - 9783037855171

VL - 529-530

T3 - Key Engineering Materials

SP - 183

EP - 186

BT - Key Engineering Materials

ER -