In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel

Jun Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Takayoshi Nakano, Takuya Ishimoto, Taiji Sohmura, Hirofumi Yatani

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Tendon insertions show specific three-dimensional (3D) cell and matrix configurations. To fabricate this transitional tissue in vitro, the development of a cell manipulation technique that can control cell and matrix patterning in the 3D construct is crucial. Bone marrow-derived stromal cells and osteoblasts were respectively cultured in fibrin gel using a custom-made device that can apply uniaxial continuous tensile strain to the gel at different strain rates. Cells in the strained fibrin gel showed a specific orientation, which was parallel to the strain direction, because of the structural change of fibrin fibres within the strained gel. The direction of cell proliferation in the strained gel was also restricted to the same direction. Subsequently, linearly aligned cell sets, similar to the cell patterning of tendon tissue, were obtained in this culture system. Notably, linearly aligned extracellular matrix and mineral patterning, which are crucial for the fabrication of tendon insertions, were also confirmed in the strained fibrin gel. A cell proliferation assay indicated that the strained gel with a higher strain rate enhanced cell proliferation in the gel. In contrast, a Real-Time PCR study indicated that the strained gel with a lower strain rate enhanced cell differentiation in the gel. Thus, our new cell culture system enabled us to fabricate a 3D construct containing patterned cells and matrices in a controllable manner. This would be an effective tool for reproducing tendon insertions in vitro.

Original languageEnglish
Pages (from-to)1662-1667
Number of pages6
JournalSoft Matter
Volume6
Issue number8
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

fibrin
Fibrin
Gels
engineering
gels
Tissue
tendons
cells
Tendons
Cell proliferation
strain rate
Strain rate
insertion
matrices
osteoblasts
Time and motion study
bone marrow
Tensile strain
Osteoblasts
Cell culture

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel. / Sasaki, Jun Ichi; Matsumoto, Takuya; Egusa, Hiroshi; Nakano, Takayoshi; Ishimoto, Takuya; Sohmura, Taiji; Yatani, Hirofumi.

In: Soft Matter, Vol. 6, No. 8, 2010, p. 1662-1667.

Research output: Contribution to journalArticle

Sasaki, JI, Matsumoto, T, Egusa, H, Nakano, T, Ishimoto, T, Sohmura, T & Yatani, H 2010, 'In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel', Soft Matter, vol. 6, no. 8, pp. 1662-1667. https://doi.org/10.1039/b922418a
Sasaki, Jun Ichi ; Matsumoto, Takuya ; Egusa, Hiroshi ; Nakano, Takayoshi ; Ishimoto, Takuya ; Sohmura, Taiji ; Yatani, Hirofumi. / In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel. In: Soft Matter. 2010 ; Vol. 6, No. 8. pp. 1662-1667.
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