In vitro degradation of hydroxyapatite nanoparticle-coated biodegradable microspheres

Syuji Fujii, Yuki Miyanari, Taiki Nishimura, Yuichi Yokoyama, Sho Hamasaki, Masahiro Okada, Tsutomu Furuzono, Shojiro Matsuda, Hideki Takamori, Yoshinobu Nakamura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In vitro degradation behavior of hydroxyapatite (HAp) nanoparticle-coated poly(l-lactide-co-ε-caprolactone) (PLCL) microspheres, which were fabricated via 'Pickering emulsion' route, has been examined in terms of weight, molecular weight, thermal property, and morphological changes through incubation in a phosphate buffered saline up to 48 weeks. Gel permeation chromatography, scanning electron microscopy (SEM) and gravimetrical method were employed to characterize their degradation profiles. It was found that molecular weight of PLCL decreased rapidly after immersing the microspheres in the buffered saline and, on the other hand, the weight of the microspheres started to decrease after 16 weeks. These results support bulk degradation for the HAp-coated PLCL microspheres. During the degradation, the enthalpy of melting increased progressively, which should be due to the increase of crystallinity because of chain rearrangement of amorphous region induced by water uptake and the preferential degradation of amorphous regions. The microspheres retained spherical morphology at least for 48 weeks and the HAp nanoparticles detached from the microsphere surface, which was confirmed by SEM study. The microspheres were easy to be broken by external pressure when the molecular weight became below 35,000 g/mol after 24-week incubation.

Original languageEnglish
Pages (from-to)377-386
Number of pages10
JournalPolymer Degradation and Stability
Volume98
Issue number1
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

Fingerprint

Durapatite
Microspheres
Hydroxyapatite
degradation
Nanoparticles
Degradation
nanoparticles
molecular weight
Molecular weight
scanning electron microscopy
gel chromatography
emulsions
Scanning electron microscopy
crystallinity
phosphates
Gel permeation chromatography
thermodynamic properties
enthalpy
routes
melting

Keywords

  • In vitro degradation
  • Microsphere
  • Molecular weight
  • Morphology
  • Poly(l-lactide-co-ε-caprolactone)

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry
  • Mechanics of Materials
  • Condensed Matter Physics

Cite this

In vitro degradation of hydroxyapatite nanoparticle-coated biodegradable microspheres. / Fujii, Syuji; Miyanari, Yuki; Nishimura, Taiki; Yokoyama, Yuichi; Hamasaki, Sho; Okada, Masahiro; Furuzono, Tsutomu; Matsuda, Shojiro; Takamori, Hideki; Nakamura, Yoshinobu.

In: Polymer Degradation and Stability, Vol. 98, No. 1, 01.2013, p. 377-386.

Research output: Contribution to journalArticle

Fujii, S, Miyanari, Y, Nishimura, T, Yokoyama, Y, Hamasaki, S, Okada, M, Furuzono, T, Matsuda, S, Takamori, H & Nakamura, Y 2013, 'In vitro degradation of hydroxyapatite nanoparticle-coated biodegradable microspheres', Polymer Degradation and Stability, vol. 98, no. 1, pp. 377-386. https://doi.org/10.1016/j.polymdegradstab.2012.09.003
Fujii, Syuji ; Miyanari, Yuki ; Nishimura, Taiki ; Yokoyama, Yuichi ; Hamasaki, Sho ; Okada, Masahiro ; Furuzono, Tsutomu ; Matsuda, Shojiro ; Takamori, Hideki ; Nakamura, Yoshinobu. / In vitro degradation of hydroxyapatite nanoparticle-coated biodegradable microspheres. In: Polymer Degradation and Stability. 2013 ; Vol. 98, No. 1. pp. 377-386.
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