In vitro cytopathology and pathogenicity to inbred mice shown by five variants of a laboratory strain of type 1 herpes simplex virus

Masao Yamada, F. Uno, S. Nii

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Abstract

The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type I herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr and -GCf]. The size of plaques produced by the five variants was found to be in the order of +GC (LPV)>+GC (81)>+GC (SPV)>-GCf>-GCr. The pathogenicity of these variants was compared in three kinds of inbred mice (AKR, C 3 H/He and C 57 BL) after intraperitoneal (IP) or intracerebral (IC) inoculation. The +GC (LPV) variant was the most virulent as shown by the highest mortality of mice by either route of inoculation. The other four variants caused death of mice only after IC inoculation, and among these variants, +GC (81) was shown to be the most virulent. These data indicate that so far as these five variants of the Miyama strain of HSV-1 are concerned, neurovirulence is positively correlated with their cell fusion activity or the size of plaques which they produce. Pre-IP-inoculation with any of the less virulent variants [-GCr, +GC (SPV) and +GC (81)] protected mice from subsequent lethal infection with +GC (LPV) by the same route of inoculation.

Original languageEnglish
Pages (from-to)183-196
Number of pages14
JournalArchives of Virology
Volume90
Issue number3-4
DOIs
Publication statusPublished - Sep 1986

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Human Herpesvirus 1
Virulence
Inbred AKR Mouse
Cell Fusion
Mortality
In Vitro Techniques
Infection

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

In vitro cytopathology and pathogenicity to inbred mice shown by five variants of a laboratory strain of type 1 herpes simplex virus. / Yamada, Masao; Uno, F.; Nii, S.

In: Archives of Virology, Vol. 90, No. 3-4, 09.1986, p. 183-196.

Research output: Contribution to journalArticle

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