In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes

Yuki Shirosaki, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka, Maria Ascensão Lopes, José Domingos Santos, Maria Helena Fernandes

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Chitosan-silicate hybrids were synthesized using γ- glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and 29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.

Original languageEnglish
Pages (from-to)485-493
Number of pages9
JournalBiomaterials
Volume26
Issue number5
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Chitosan
Membranes
Calcium
Cells
Silicates
Cell adhesion
Cultured Cells
Nuclear magnetic resonance spectroscopy
Cell Proliferation
Fourier transform infrared spectroscopy
Adhesion
In Vitro Techniques
Ions
Fourier Transform Infrared Spectroscopy
Cell Adhesion
Cell Differentiation
Magnetic Resonance Spectroscopy
Cell Line

Keywords

  • γ-Glycidoxypropyltrimethoxysilane (GPSM)
  • Chitosan
  • Cytocompatibility
  • MG63 osteoblastic cells
  • Organic-inorganic hybrid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Shirosaki, Y., Tsuru, K., Hayakawa, S., Osaka, A., Lopes, M. A., Santos, J. D., & Fernandes, M. H. (2005). In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes. Biomaterials, 26(5), 485-493. https://doi.org/10.1016/j.biomaterials.2004.02.056

In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes. / Shirosaki, Yuki; Tsuru, Kanji; Hayakawa, Satoshi; Osaka, Akiyoshi; Lopes, Maria Ascensão; Santos, José Domingos; Fernandes, Maria Helena.

In: Biomaterials, Vol. 26, No. 5, 02.2005, p. 485-493.

Research output: Contribution to journalArticle

Shirosaki, Y, Tsuru, K, Hayakawa, S, Osaka, A, Lopes, MA, Santos, JD & Fernandes, MH 2005, 'In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes', Biomaterials, vol. 26, no. 5, pp. 485-493. https://doi.org/10.1016/j.biomaterials.2004.02.056
Shirosaki, Yuki ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Lopes, Maria Ascensão ; Santos, José Domingos ; Fernandes, Maria Helena. / In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes. In: Biomaterials. 2005 ; Vol. 26, No. 5. pp. 485-493.
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