Abstract
Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day- old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.
| Original language | English |
|---|---|
| Pages (from-to) | 245-251 |
| Number of pages | 7 |
| Journal | Experimental Cell Research |
| Volume | 245 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Dec 15 1998 |
| Externally published | Yes |
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Keywords
- Ascorbic acid
- bFGF
- Iris-pigmented epithelial cells
- Lens transdifferentiation
- Pax6
ASJC Scopus subject areas
- Cell Biology
Cite this
In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation. / Kosaka, Mitsuko; Kodama, Ryuji; Eguchi, Goro.
In: Experimental Cell Research, Vol. 245, No. 2, 15.12.1998, p. 245-251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation
AU - Kosaka, Mitsuko
AU - Kodama, Ryuji
AU - Eguchi, Goro
PY - 1998/12/15
Y1 - 1998/12/15
N2 - Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day- old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.
AB - Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day- old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.
KW - Ascorbic acid
KW - bFGF
KW - Iris-pigmented epithelial cells
KW - Lens transdifferentiation
KW - Pax6
UR - http://www.scopus.com/inward/record.url?scp=0032534467&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032534467&partnerID=8YFLogxK
U2 - 10.1006/excr.1998.4211
DO - 10.1006/excr.1998.4211
M3 - Article
VL - 245
SP - 245
EP - 251
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -