In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation

Mitsuko Kosaka, Ryuji Kodama, Goro Eguchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day- old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalExperimental Cell Research
Volume245
Issue number2
DOIs
Publication statusPublished - Dec 15 1998
Externally publishedYes

Fingerprint

Iris
Epithelial Cells
Lenses
Epithelium
Northern Blotting
Ascorbic Acid
Culture Media
Regeneration
Up-Regulation
In Vitro Techniques
Genes

Keywords

  • Ascorbic acid
  • bFGF
  • Iris-pigmented epithelial cells
  • Lens transdifferentiation
  • Pax6

ASJC Scopus subject areas

  • Cell Biology

Cite this

In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation. / Kosaka, Mitsuko; Kodama, Ryuji; Eguchi, Goro.

In: Experimental Cell Research, Vol. 245, No. 2, 15.12.1998, p. 245-251.

Research output: Contribution to journalArticle

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