TY - JOUR
T1 - In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation
AU - Kosaka, Mitsuko
AU - Kodama, Ryuji
AU - Eguchi, Goro
N1 - Funding Information:
We greatly appreciate the skillful technical assistance of Ms. H. Urai and Ms. C. Takagi. We also thank Dr. M. Mochii for providing some cDNA probes prior to publication and for his helpful discussions. This work is supported by Grants-in-Aid from the Ministry of Education, Science and Culture to G.E., R.K., and M.K. and by Japan Science and Technology Corporation to M.K.
PY - 1998/12/15
Y1 - 1998/12/15
N2 - Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day- old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.
AB - Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day- old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.
KW - Ascorbic acid
KW - Iris-pigmented epithelial cells
KW - Lens transdifferentiation
KW - Pax6
KW - bFGF
UR - http://www.scopus.com/inward/record.url?scp=0032534467&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032534467&partnerID=8YFLogxK
U2 - 10.1006/excr.1998.4211
DO - 10.1006/excr.1998.4211
M3 - Article
C2 - 9851864
AN - SCOPUS:0032534467
VL - 245
SP - 245
EP - 251
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -