In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel

Jae Won Lee, Hyun Seung Kim, Soo Jeong Yon, Takuya Matsumoto, Sang Kyung Lee, Kuen Yong Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Stem cells exist and maintain their quiescence and pluripotency in stem cell niche. Here, we hypothesized that regulation of cell-cell interactions using a polymeric scaffold as synthetic extracellular matrix (ECM) could be critical in creating a hematopoietic stem cell (HSC) niche in vitro. Angiopoietin-1 (Ang1) binds to the tyrosine kinase receptor (Tie2), and regulation of the Tie2/Ang1 interaction is important in maintaining the quiescence of HSCs in vivo. Alginate hydrogel was thus modified with Ang1 as a synthetic ECM to mimic the HSC niche. Long-term HSCs (CD34, CD135, and CD150+) were isolated from mouse femurs and cultured on Ang1-modified alginate hydrogel. The percentage of LT-HSCs in G0 phase was 46.8 ± 1.8%, which was comparable to that of LT-HSCs co-cultured with osteoblasts (46.8 ± 2.1%). Ang1-coupled alginate gels were useful to provide a niche for HSC quiescence without a co-culture system. Polymeric scaffolds containing biomimetic and cell-instructive characteristics for stem cell phenotype regulation might help create HSC niches in vitro and be useful to engineer tissues and transplant stem cells.

Original languageEnglish
Pages (from-to)1893-1899
Number of pages7
JournalInternational Journal of Biological Macromolecules
Volume209
DOIs
Publication statusPublished - Jun 1 2022
Externally publishedYes

Keywords

  • Alginate hydrogel
  • Angiopoietin-1
  • Hematopoietic stem cell
  • Synthetic niche

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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