In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO 2: HA formation, cellular affinity and organ culture

Sang Hyun An, Takuya Matsumoto, Jun Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo Han Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Anodization is an easily viable technique useful for producing TiO 2 coatings on titanium substrates. Nano-crystalline anodic TiO 2 structure was produced on titanium at 20 V using 1 M Na 2SO 4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO 2 was produced on titanium by applying a galvanostatic current density of 70 A/m 2 in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface.

Original languageEnglish
Pages (from-to)2516-2522
Number of pages7
JournalMaterials Science and Engineering C
Volume32
Issue number8
DOIs
Publication statusPublished - Dec 1 2012
Externally publishedYes

Fingerprint

Durapatite
Bioactivity
Hydroxyapatite
organs
Oxides
affinity
Crystalline materials
oxides
Titanium
evaluation
titanium
Bone
bones
mice
Body fluids
Osteoblasts
osteoblasts
body fluids
Cell culture
Current density

Keywords

  • Anodization
  • In vitro study
  • Micro-surface
  • Nano-surface
  • Surface modification

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO 2 : HA formation, cellular affinity and organ culture. / An, Sang Hyun; Matsumoto, Takuya; Sasaki, Jun Ichi; Miyajima, Hiroyuki; Narayanan, Ramaswamy; Imazato, Satoshi; Kim, Kyo Han.

In: Materials Science and Engineering C, Vol. 32, No. 8, 01.12.2012, p. 2516-2522.

Research output: Contribution to journalArticle

An, Sang Hyun ; Matsumoto, Takuya ; Sasaki, Jun Ichi ; Miyajima, Hiroyuki ; Narayanan, Ramaswamy ; Imazato, Satoshi ; Kim, Kyo Han. / In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO 2 : HA formation, cellular affinity and organ culture. In: Materials Science and Engineering C. 2012 ; Vol. 32, No. 8. pp. 2516-2522.
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