Abstract
Anodization is an easily viable technique useful for producing TiO 2 coatings on titanium substrates. Nano-crystalline anodic TiO 2 structure was produced on titanium at 20 V using 1 M Na 2SO 4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO 2 was produced on titanium by applying a galvanostatic current density of 70 A/m 2 in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface.
| Original language | English |
|---|---|
| Pages (from-to) | 2516-2522 |
| Number of pages | 7 |
| Journal | Materials Science and Engineering C |
| Volume | 32 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Dec 1 2012 |
| Externally published | Yes |
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Keywords
- Anodization
- In vitro study
- Micro-surface
- Nano-surface
- Surface modification
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering
- Mechanics of Materials
Cite this
In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO 2 : HA formation, cellular affinity and organ culture. / An, Sang Hyun; Matsumoto, Takuya; Sasaki, Jun Ichi; Miyajima, Hiroyuki; Narayanan, Ramaswamy; Imazato, Satoshi; Kim, Kyo Han.
In: Materials Science and Engineering C, Vol. 32, No. 8, 01.12.2012, p. 2516-2522.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO 2
T2 - HA formation, cellular affinity and organ culture
AU - An, Sang Hyun
AU - Matsumoto, Takuya
AU - Sasaki, Jun Ichi
AU - Miyajima, Hiroyuki
AU - Narayanan, Ramaswamy
AU - Imazato, Satoshi
AU - Kim, Kyo Han
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Anodization is an easily viable technique useful for producing TiO 2 coatings on titanium substrates. Nano-crystalline anodic TiO 2 structure was produced on titanium at 20 V using 1 M Na 2SO 4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO 2 was produced on titanium by applying a galvanostatic current density of 70 A/m 2 in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface.
AB - Anodization is an easily viable technique useful for producing TiO 2 coatings on titanium substrates. Nano-crystalline anodic TiO 2 structure was produced on titanium at 20 V using 1 M Na 2SO 4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO 2 was produced on titanium by applying a galvanostatic current density of 70 A/m 2 in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface.
KW - Anodization
KW - In vitro study
KW - Micro-surface
KW - Nano-surface
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=84866012232&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866012232&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2012.07.034
DO - 10.1016/j.msec.2012.07.034
M3 - Article
AN - SCOPUS:84866012232
VL - 32
SP - 2516
EP - 2522
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
IS - 8
ER -