In vitro assessment of factors affecting the apparent diffusion coefficient of ramos cells using bio-phantoms

Takanori Sasaki, Masahiro Kurod, Kazunori Katashima, Masakazu Ashida, Hidenobu Matsuzaki, Jun-Ichi Asaumi, Jun Murakami, Seiichiro Ohno, Hirokazu Kato, Susumu Kanazawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkitt's lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in wtro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalActa Medica Okayama
Volume66
Issue number3
Publication statusPublished - 2012

Fingerprint

Extracellular Space
Apoptosis
Cell Count
Cell Membrane Structures
Cells
Membranes
Sonication
Burkitt Lymphoma
In Vitro Techniques
Tumors
Reference Values
Cell Line
Pharmaceutical Preparations
Neoplasms
Rituximab

Keywords

  • Apparent diffusion coefficient value
  • Bio-phantom
  • Cell density
  • Extracellular space

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

In vitro assessment of factors affecting the apparent diffusion coefficient of ramos cells using bio-phantoms. / Sasaki, Takanori; Kurod, Masahiro; Katashima, Kazunori; Ashida, Masakazu; Matsuzaki, Hidenobu; Asaumi, Jun-Ichi; Murakami, Jun; Ohno, Seiichiro; Kato, Hirokazu; Kanazawa, Susumu.

In: Acta Medica Okayama, Vol. 66, No. 3, 2012, p. 263-270.

Research output: Contribution to journalArticle

@article{50afcddb9fa04c7fb02ddb57a553ebbc,
title = "In vitro assessment of factors affecting the apparent diffusion coefficient of ramos cells using bio-phantoms",
abstract = "The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkitt's lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65{\%} of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35{\%} originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in wtro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.",
keywords = "Apparent diffusion coefficient value, Bio-phantom, Cell density, Extracellular space",
author = "Takanori Sasaki and Masahiro Kurod and Kazunori Katashima and Masakazu Ashida and Hidenobu Matsuzaki and Jun-Ichi Asaumi and Jun Murakami and Seiichiro Ohno and Hirokazu Kato and Susumu Kanazawa",
year = "2012",
language = "English",
volume = "66",
pages = "263--270",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "3",

}

TY - JOUR

T1 - In vitro assessment of factors affecting the apparent diffusion coefficient of ramos cells using bio-phantoms

AU - Sasaki, Takanori

AU - Kurod, Masahiro

AU - Katashima, Kazunori

AU - Ashida, Masakazu

AU - Matsuzaki, Hidenobu

AU - Asaumi, Jun-Ichi

AU - Murakami, Jun

AU - Ohno, Seiichiro

AU - Kato, Hirokazu

AU - Kanazawa, Susumu

PY - 2012

Y1 - 2012

N2 - The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkitt's lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in wtro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.

AB - The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkitt's lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in wtro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.

KW - Apparent diffusion coefficient value

KW - Bio-phantom

KW - Cell density

KW - Extracellular space

UR - http://www.scopus.com/inward/record.url?scp=84864740083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864740083&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 263

EP - 270

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 3

ER -