TY - JOUR
T1 - In vitro and in vivo selection of probiotic purple nonsulphur bacteria with an ability to inhibit shrimp pathogens
T2 - acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus and other vibrios
AU - Chumpol, Supaporn
AU - Kantachote, Duangporn
AU - Rattanachuay, Pattamarat
AU - Vuddhakul, Varaporn
AU - Nitoda, Teruhiko
AU - Kanzaki, Hiroshi
N1 - Funding Information:
This work was financially supported by the Thailand Research Fund through the Royal Golden Jubilee (RGJ) Ph.D. Program, grant number PHD/0106/2554, and partly supported by the Graduate School, Prince of Songkla University. The authors would like to thank the Aquatic Animal Help Research Center (AAHRC) for providing shrimp pathogenic bacteria used in this study. The authors would also like to thank Dr Brian Hodgson for assistance with the English. The authors declared that they have no conflict of interest.
Publisher Copyright:
© 2016 John Wiley & Sons Ltd
PY - 2017/6
Y1 - 2017/6
N2 - Shrimp cultivation has been faced with huge losses in productivity caused by infectious shrimp pathogenic vibrios, especially Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (AHPND). Hence, purple nonsulphur bacteria (PNSB) were isolated from shrimp ponds for investigating their abilities to control shrimp pathogenic Vibrio spp. and their use as probiotics for sustainable shrimp cultivation. Based on their probiotic properties, strains S3W10 and SS15 were selected because of their strong abilities to produce amylase, gelatinase and vitamin B12. However, only three PNSB strains (SS15, TKW17 and STW181) strongly inhibited V. harveyi_KSAAHRC and V. vulnificus_KSAAHRC including V. parahaemolyticusAHPND strains by secreting antivibrio compounds. Four selected PNSB also grew in the presence of pancreatic enzymes, and they were identified as Rhodobacter sphaeroides for strains S3W10, SS15 and TKW17 and Afifella marina for strain STW181. The effects of a mixed culture were also investigated as follows: T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) on postlarval white shrimp (Litopenaeus vannamei) for 60 days by comparison with a control. All three probiotic PNSB sets significantly improved the digestive enzyme activities and shrimp growth with their proliferation in shrimp gastrointestinal tract although the shrimp survival was not significantly different. They also significantly reduced the cumulative mortality of shrimp exposed to a virulent AHPND strain (V. parahaemolyticusSR2). This is the first to conclude that selected probiotic PNSB strains have great potential to be used for shrimp cultivation to control vibrios including AHPND strains.
AB - Shrimp cultivation has been faced with huge losses in productivity caused by infectious shrimp pathogenic vibrios, especially Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (AHPND). Hence, purple nonsulphur bacteria (PNSB) were isolated from shrimp ponds for investigating their abilities to control shrimp pathogenic Vibrio spp. and their use as probiotics for sustainable shrimp cultivation. Based on their probiotic properties, strains S3W10 and SS15 were selected because of their strong abilities to produce amylase, gelatinase and vitamin B12. However, only three PNSB strains (SS15, TKW17 and STW181) strongly inhibited V. harveyi_KSAAHRC and V. vulnificus_KSAAHRC including V. parahaemolyticusAHPND strains by secreting antivibrio compounds. Four selected PNSB also grew in the presence of pancreatic enzymes, and they were identified as Rhodobacter sphaeroides for strains S3W10, SS15 and TKW17 and Afifella marina for strain STW181. The effects of a mixed culture were also investigated as follows: T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) on postlarval white shrimp (Litopenaeus vannamei) for 60 days by comparison with a control. All three probiotic PNSB sets significantly improved the digestive enzyme activities and shrimp growth with their proliferation in shrimp gastrointestinal tract although the shrimp survival was not significantly different. They also significantly reduced the cumulative mortality of shrimp exposed to a virulent AHPND strain (V. parahaemolyticusSR2). This is the first to conclude that selected probiotic PNSB strains have great potential to be used for shrimp cultivation to control vibrios including AHPND strains.
KW - Vibrio spp.
KW - antivibrio compounds
KW - early mortality syndrome
KW - probiotics
KW - purple nonsulphur bacteria
KW - shrimp cultivation
UR - http://www.scopus.com/inward/record.url?scp=84994302351&partnerID=8YFLogxK
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U2 - 10.1111/are.13149
DO - 10.1111/are.13149
M3 - Article
AN - SCOPUS:84994302351
VL - 48
SP - 3182
EP - 3197
JO - Aquaculture Research
JF - Aquaculture Research
SN - 1355-557X
IS - 6
ER -