In vitro and in vivo evaluation of three newly isolated bacteriophage candidates, phief7h, phief14h1, phief19g, for treatment of enterococcus faecalis endophthalmitis

Tatsuma Kishimoto, Waka Ishida, Tadahiro Nasukawa, Takako Ujihara, Isana Nakajima, Takashi Suzuki, Jumpei Uchiyama, Daisuke Todokoro, Masanori Daibata, Atsuki Fukushima, Shigenobu Matsuzaki, Ken Fukuda

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Post-operative endophthalmitis caused by Enterococcus spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an optional therapy for infectious diseases. Therefore, we investigated the therapeutic potential of three newly isolated enterococcal phages, phiEF7H, phiEF14H1, and phiEF19G, in E. fae-calis-induced endophthalmitis. These phages could lyse the broad-range E. faecalis, including strains derived from endophthalmitis and vancomycin-resistant E. faecalis in vitro, as determined by the streak test. Morphological and genomic analyses revealed that these phages were classified into the Herelleviridae genus Kochikohdavirus. The whole genomes of these phages contained 143,399, 143,280, and 143,400 bp, respectively. Endophthalmitis was induced in mice by injection of three strains of E. faecalis derived from post-operative endophthalmitis or vancomycin-resistant strains into the vit-reous body. The number of viable bacteria and infiltration of neutrophils in the eye were both de-creased by intravitreous injection of phiEF7H, phiEF14H1, and phiEF19G 6 h after injection of all E. faecalis strains. Thus, these results suggest that these newly isolated phages may serve as promising candidates for phage therapy against endophthalmitis.

Original languageEnglish
Article number212
Pages (from-to)1-12
Number of pages12
JournalMicroorganisms
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 2021
Externally publishedYes

Keywords

  • Bacteriophage
  • Endophthalmitis
  • Enterococcus faecalis
  • Herelleviridae
  • Mouse model
  • Phage therapy

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Virology

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