In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells

Tetsuro Mazaki, Takashi Kitajima, Yasuyuki Shiozaki, Miwa Sato, Megumi Mino, Aki Yoshida, Mariko Nakamura, Yasuhiro Yoshida, Masato Tanaka, Toshifumi Ozaki, Akihiro Matsukawa, Yoshihiro Ito

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

High-throughput screening was performed as a phenotypic screening on clinically relevant human mesenchymal stem cells to identify small molecules that affect stem cell fate. From a library of pharmacologically active small molecules, we identified kaempferol, which is a natural flavonol, with a high osteogenic activity. Kaempferol achieved the highest levels of bone-specific markers among the chemicals tested in vitro. The osteogenic activity of kaempferol was the same as that of ipriflavone, which is a synthetic isoflavone, in ovariectomized mouse experiments. Considering that kaempferol is present in various foods such as vegetables and fruits, it will be useful as a new preventive medicine for osteoporosis.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalJournal of Functional Foods
Volume6
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Flavonoids
  • High-throughput screening
  • Ipriflavone
  • Kaempferol
  • Mesenchymal stem cell
  • Osteoporosis

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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    Mazaki, T., Kitajima, T., Shiozaki, Y., Sato, M., Mino, M., Yoshida, A., Nakamura, M., Yoshida, Y., Tanaka, M., Ozaki, T., Matsukawa, A., & Ito, Y. (2014). In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells. Journal of Functional Foods, 6(1), 241-247. https://doi.org/10.1016/j.jff.2013.10.013