In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route

Michiyo Honda, Yusuke Kawanobe, Ken Ishii, Toshiisa Konishi, Minori Mizumoto, Nobuyuki Kanzawa, Morio Matsumoto, Mamoru Aizawa

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Hydroxyapatite (HAp), with its high biocompatibility and osteoconductivity, readily absorbs proteins, amino acids and other substances, which in turn favor the adsorption and colonization of bacteria. To prevent bacterial growth and biofilm formation on HAp discs, silver-containing (1-20 mol%) HAp (Ag-HAp) powders were synthesized using an ultrasonic spray pyrolysis (USSP) technique. The X-ray diffraction (XRD) peaks were very broad, indicating low crystallinity, and this induced the release of Ag+ ions from Ag-HAp powders. In addition, a gradual increase in Ca2+ ion release was observed. These results suggest that dissolution of Ca2+ ion in Ag-HAp triggered the release of Ag+ ions. The antimicrobial efficacy of Ag-HAp disc was tested against Staphylococcus aureus. Samples with Ag contents of more than 5 mol%were found to be highly effective against bacterial colonization and biofilm formation in vitro. In vivo antibacterial tests using bioluminescent strains also showed reductions in the viability of bacteria with Ag-HAp (5 mol%) discs. Biocompatibility tests using a modified Transwell® insert method showed that Ag-HAp (5 mol%) discs have negative effects on osteoblast proliferation. These results indicate that Ag-HAp (5 mol%) has effective antibacterial activity and good biocompatibility both in vitro and in vivo together with good biocompatibility, thus confirming its utility as a bactericidal material.

Original languageEnglish
Pages (from-to)5008-5018
Number of pages11
JournalMaterials Science and Engineering C
Volume33
Issue number8
DOIs
Publication statusPublished - Dec 1 2013
Externally publishedYes

Fingerprint

Spray pyrolysis
biocompatibility
Durapatite
Hydroxyapatite
Silver
Ultrasonics
pyrolysis
sprayers
ultrasonics
silver
routes
biofilms
bacteria
ions
Biocompatibility
osteoblasts
staphylococcus
Ions
inserts
viability

Keywords

  • Antibacterial effect
  • Biofilm resistance
  • Implant-related infection
  • Silver-containing hydroxyapatite

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials
  • Medicine(all)

Cite this

In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route. / Honda, Michiyo; Kawanobe, Yusuke; Ishii, Ken; Konishi, Toshiisa; Mizumoto, Minori; Kanzawa, Nobuyuki; Matsumoto, Morio; Aizawa, Mamoru.

In: Materials Science and Engineering C, Vol. 33, No. 8, 01.12.2013, p. 5008-5018.

Research output: Contribution to journalArticle

Honda, M, Kawanobe, Y, Ishii, K, Konishi, T, Mizumoto, M, Kanzawa, N, Matsumoto, M & Aizawa, M 2013, 'In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route', Materials Science and Engineering C, vol. 33, no. 8, pp. 5008-5018. https://doi.org/10.1016/j.msec.2013.08.026
Honda, Michiyo ; Kawanobe, Yusuke ; Ishii, Ken ; Konishi, Toshiisa ; Mizumoto, Minori ; Kanzawa, Nobuyuki ; Matsumoto, Morio ; Aizawa, Mamoru. / In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route. In: Materials Science and Engineering C. 2013 ; Vol. 33, No. 8. pp. 5008-5018.
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