In situ potentiometric method to evaluate bacterial outer membrane-permeabilizing ability of drugs: Example using antiprotozoal diamidines

Makoto Ando, Rie Kamei, Keiko Komagoe, Tsuyoshi Inoue, Keiichi Yamada, Takashi Katsu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We introduced a new assay system, combining tyrocidine A and a K+-selective electrode, to evaluate the bacterial outer membrane-permeabilizing ability of drugs. Tyrocidine A, in the presence of an outer membrane permeabilizer, increased the permeability to K+ of the cytoplasmic membrane of Escherichia coli, because this antibiotic could markedly increase the permeability of phospholipid layers constituting the cytoplasmic membrane, while it acted weakly on the outer membrane. Hence, the novel function of agents increasing the permeability of the outer membrane could be examined directly by monitoring the tyrocidine A-induced leakage of K+ from the bacterial cytoplasm using a K+-selective electrode. We found that antiprotozoal diamidines, such as diminazene, pentamidine, and 4',6-diamidino-2-phenylindole (DAPI), can increase the permeability of the bacterial outer membrane and appropriate lipophilicity is important for diamidines to permeabilize the outer membrane.

Original languageEnglish
Pages (from-to)497-500
Number of pages4
JournalJournal of Microbiological Methods
Volume91
Issue number3
DOIs
Publication statusPublished - Dec 1 2012

Keywords

  • Bacterial outer membrane permeabilizer
  • DAPI
  • Diminazene
  • K-selective electrode
  • Pentamidine
  • Tyrocidine A

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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