In situ monitoring of photodynamic inactivation of the membrane functions of bacteria using electrochemical sensors

Hisato Kato, Keiko Komagoe, Tsuyoshi Inoue, Takashi Katsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The photodynamic inactivation of the membrane functions of bacteria was analyzed in situ, using K+ and tetraphenylphosphonium (TPP+) electrodes, as well as an oxygen electrode. Tetrakis(4-N-trimethylaminophenyl)porphine (TTMAPP) and rose bengal were used, since both dyes act strongly on bacteria, such as Staphylococcus aureus. After a short time lag, they inhibited the respiration of bacteria and increased the permeability of the cytoplasmic membrane to K+, while dissipating the membrane potential. This combination of sensors is quite useful for visualizing the actions of photosensitizers on the bacterial membrane. TTMAPP and rose bengal impaired the bacterial function by reducing the membrane potential within minutes of photo-irradiation.

Original languageEnglish
Pages (from-to)1019-1021
Number of pages3
JournalAnalytical Sciences
Volume26
Issue number10
DOIs
Publication statusPublished - 2010

Fingerprint

Electrochemical sensors
Bacteria
Membranes
Monitoring
Rose Bengal
Electrodes
Photosensitizing Agents
Coloring Agents
Irradiation
Oxygen
Sensors

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

In situ monitoring of photodynamic inactivation of the membrane functions of bacteria using electrochemical sensors. / Kato, Hisato; Komagoe, Keiko; Inoue, Tsuyoshi; Katsu, Takashi.

In: Analytical Sciences, Vol. 26, No. 10, 2010, p. 1019-1021.

Research output: Contribution to journalArticle

@article{785045469dab4e498e6fdc20cb3b796a,
title = "In situ monitoring of photodynamic inactivation of the membrane functions of bacteria using electrochemical sensors",
abstract = "The photodynamic inactivation of the membrane functions of bacteria was analyzed in situ, using K+ and tetraphenylphosphonium (TPP+) electrodes, as well as an oxygen electrode. Tetrakis(4-N-trimethylaminophenyl)porphine (TTMAPP) and rose bengal were used, since both dyes act strongly on bacteria, such as Staphylococcus aureus. After a short time lag, they inhibited the respiration of bacteria and increased the permeability of the cytoplasmic membrane to K+, while dissipating the membrane potential. This combination of sensors is quite useful for visualizing the actions of photosensitizers on the bacterial membrane. TTMAPP and rose bengal impaired the bacterial function by reducing the membrane potential within minutes of photo-irradiation.",
author = "Hisato Kato and Keiko Komagoe and Tsuyoshi Inoue and Takashi Katsu",
year = "2010",
doi = "10.2116/analsci.26.1019",
language = "English",
volume = "26",
pages = "1019--1021",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Japan Society for Analytical Chemistry",
number = "10",

}

TY - JOUR

T1 - In situ monitoring of photodynamic inactivation of the membrane functions of bacteria using electrochemical sensors

AU - Kato, Hisato

AU - Komagoe, Keiko

AU - Inoue, Tsuyoshi

AU - Katsu, Takashi

PY - 2010

Y1 - 2010

N2 - The photodynamic inactivation of the membrane functions of bacteria was analyzed in situ, using K+ and tetraphenylphosphonium (TPP+) electrodes, as well as an oxygen electrode. Tetrakis(4-N-trimethylaminophenyl)porphine (TTMAPP) and rose bengal were used, since both dyes act strongly on bacteria, such as Staphylococcus aureus. After a short time lag, they inhibited the respiration of bacteria and increased the permeability of the cytoplasmic membrane to K+, while dissipating the membrane potential. This combination of sensors is quite useful for visualizing the actions of photosensitizers on the bacterial membrane. TTMAPP and rose bengal impaired the bacterial function by reducing the membrane potential within minutes of photo-irradiation.

AB - The photodynamic inactivation of the membrane functions of bacteria was analyzed in situ, using K+ and tetraphenylphosphonium (TPP+) electrodes, as well as an oxygen electrode. Tetrakis(4-N-trimethylaminophenyl)porphine (TTMAPP) and rose bengal were used, since both dyes act strongly on bacteria, such as Staphylococcus aureus. After a short time lag, they inhibited the respiration of bacteria and increased the permeability of the cytoplasmic membrane to K+, while dissipating the membrane potential. This combination of sensors is quite useful for visualizing the actions of photosensitizers on the bacterial membrane. TTMAPP and rose bengal impaired the bacterial function by reducing the membrane potential within minutes of photo-irradiation.

UR - http://www.scopus.com/inward/record.url?scp=77957920826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957920826&partnerID=8YFLogxK

U2 - 10.2116/analsci.26.1019

DO - 10.2116/analsci.26.1019

M3 - Article

C2 - 20953042

AN - SCOPUS:77957920826

VL - 26

SP - 1019

EP - 1021

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 10

ER -