In Situ Localization of Rat Testis-Specific Calcineurin B Subunit Isoform β1 in the Developing Rat Testis

K. Miyamoto, Hideki Matsui, Kazuhito Tomizawa, Y. Kuwata, T. Itano, M. Tokuda, O. Hatase

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In situ localization and developmental changes in expression of testis-specific calcineurin B subunit isoform β1 was examined in rat testis. Two different sizes of mRNA signal, 4.0 kb and 0.9 kb, were detected by Northern blot hybridization. Both signals were expressed synchronously with the start of meiosis at 3 weeks after birth, and increased depending on the maturation of spermatogenesis. In situ hybridization using non-radioactive riboprobes showed that the β1 mRNA was specifically localized to spermatocytes where meiosis occurs but none or very little was observed in spermatogonia, spermatids, Sertoli or Leydig cells.

Original languageEnglish
Pages (from-to)1275-1283
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number2
DOIs
Publication statusPublished - Sep 15 1994
Externally publishedYes

Fingerprint

Calcineurin
Meiosis
Testis
Rats
Protein Isoforms
Messenger RNA
Spermatogonia
Spermatocytes
Spermatids
Leydig Cells
Sertoli Cells
Spermatogenesis
Northern Blotting
In Situ Hybridization
Parturition

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

In Situ Localization of Rat Testis-Specific Calcineurin B Subunit Isoform β1 in the Developing Rat Testis. / Miyamoto, K.; Matsui, Hideki; Tomizawa, Kazuhito; Kuwata, Y.; Itano, T.; Tokuda, M.; Hatase, O.

In: Biochemical and Biophysical Research Communications, Vol. 203, No. 2, 15.09.1994, p. 1275-1283.

Research output: Contribution to journalArticle

Miyamoto, K. ; Matsui, Hideki ; Tomizawa, Kazuhito ; Kuwata, Y. ; Itano, T. ; Tokuda, M. ; Hatase, O. / In Situ Localization of Rat Testis-Specific Calcineurin B Subunit Isoform β1 in the Developing Rat Testis. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 203, No. 2. pp. 1275-1283.
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