In situ localization of rat testis-specific calcineurin B subunit isoform β1 in the developing rat testis

Kazuhiro Miyamoto, Hideki Matsui, Kazuhito Tomizawa, Yoshihiro Kuwata, Toshifumi Itano, Masaaki Tokuda, Osamu Hatase

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In situ localization and developmental changes in expression of testis-specific calcineurin B subunit isoform β1 was examined in rat testis. Two different sizes of mRNA signal, 4.0 kb and 0.9 kb, were detected by Northern blot hybridization. Both signals were expressed synchronously with the start of meiosis at 3 weeks after birth, and increased depending on the maturation of spermatogenesis. In situ hybridization using non-radioactive riboprobes showed that the β1 mRNA was specifically localized to spermatocytes where meiosis occurs but none or very little was observed in spermatogonia, spermatids, Sertoli or Leydig cells.

Original languageEnglish
Pages (from-to)1275-1283
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number2
DOIs
Publication statusPublished - Sep 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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