In situ localization and developmental changes in expression of testis-specific calcineurin B subunit isoform β1 was examined in rat testis. Two different sizes of mRNA signal, 4.0 kb and 0.9 kb, were detected by Northern blot hybridization. Both signals were expressed synchronously with the start of meiosis at 3 weeks after birth, and increased depending on the maturation of spermatogenesis. In situ hybridization using non-radioactive riboprobes showed that the β1 mRNA was specifically localized to spermatocytes where meiosis occurs but none or very little was observed in spermatogonia, spermatids, Sertoli or Leydig cells.
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Sep 15 1994|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology