In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired

Suleyman Allakhverdiev, Eva Šetlíková, Vyacheslav V. Klimov, Ivan Šetlík

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Oxygen-evolving photosystem II particles (DT 20) isolated from pea chloroplasts by digitonin-Triton X-100 fractionation were photoinhibited with 150 W·m-2 white light, at 20°C under three conditions: aerobic, anaerobic and strongly reducing (Eh poised to approx. -250 mV with dithionite). Hill reaction rate (H2O → BQ) and variable fluorescence (Fv) declined in parallel in all three cases with shortening half times: 30, 10 and 2.5 min, respectively. Light-induced absorbance changes at 685 nm characteristic of reversible photoaccumulation of reduced pheophytin (Eh ≈ -250 mV) remained essentially unchanged. We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center.

Original languageEnglish
Pages (from-to)186-190
Number of pages5
JournalFEBS Letters
Volume226
Issue number1
DOIs
Publication statusPublished - Dec 21 1987
Externally publishedYes

Fingerprint

Pheophytins
Photosystem II Protein Complex
Dithionite
Light
Digitonin
Peas
Octoxynol
Chloroplasts
Fractionation
Reaction rates
Fluorescence
Oxygen

Keywords

  • (Pea chloroplasts)
  • Charge separation
  • Oxygen-evolving PS II particle
  • Pheophytin
  • Photoinhibition

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Allakhverdiev, S., Šetlíková, E., Klimov, V. V., & Šetlík, I. (1987). In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired. FEBS Letters, 226(1), 186-190. https://doi.org/10.1016/0014-5793(87)80576-8

In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired. / Allakhverdiev, Suleyman; Šetlíková, Eva; Klimov, Vyacheslav V.; Šetlík, Ivan.

In: FEBS Letters, Vol. 226, No. 1, 21.12.1987, p. 186-190.

Research output: Contribution to journalArticle

Allakhverdiev, S, Šetlíková, E, Klimov, VV & Šetlík, I 1987, 'In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired', FEBS Letters, vol. 226, no. 1, pp. 186-190. https://doi.org/10.1016/0014-5793(87)80576-8
Allakhverdiev, Suleyman ; Šetlíková, Eva ; Klimov, Vyacheslav V. ; Šetlík, Ivan. / In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired. In: FEBS Letters. 1987 ; Vol. 226, No. 1. pp. 186-190.
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