Improvements in the measurement of stool decay-accelerating factor in the detection of colorectal cancer

Shogen Ohya, Motowo Mizuno, Mikihiro Kawada, Junichirou Nasu, Hiroyuki Okada, Hiroyuki Shimomura, Kazuhide Yamamoto, Teizo Fujita, Takao Tsuji

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10 Citations (Scopus)

Abstract

We have previously developed an enzyme-linked immunosorbent assay (ELISA) to measure stool decay-accelerating factor (DAF) and found that stool DAF concentrations were significantly elevated in patients with colorectal cancer, suggesting that the measurement of stool DAF may be valuable for the detection of colorectal cancer. In order to refine the assay for the measurement of stool DAF, we investigated 1) effects of centrifugation of stool samples, 2) effects of detergents, and 3) adequate combination of various anti-DAF monoclonal antibodies for the ELISA system using only monoclonal antibodies. We found that high-speed centrifugation could be omitted and that only the removal of large undigested food residues by centrifugation of short duration in a low-speed benchtop microcentrifuge sufficed to adequately prepare the stool samples. Addition of 2 detergents, octyl β-glucoside and sodium deoxycholate, known to solubilize glycosyl-phosphatidylinositol-anchored proteins such as DAF, did not influence stool DAF values. By using 2 mouse anti-DAF monoclonal antibodies (clone 4F11 and 1C6), we were able to achieve a stable ELISA for the measurement of stool DAF using a uniform source of antibodies. The results should allow us to consistently apply the DAF assay for routine use in the detection of colorectal cancer.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalActa Medica Okayama
Volume56
Issue number4
Publication statusPublished - 2002

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Keywords

  • Colorectal cancer
  • Decay-accelerating factor (DAF)
  • Enzyme-linked immunosorbent assay (ELISA)
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ohya, S., Mizuno, M., Kawada, M., Nasu, J., Okada, H., Shimomura, H., Yamamoto, K., Fujita, T., & Tsuji, T. (2002). Improvements in the measurement of stool decay-accelerating factor in the detection of colorectal cancer. Acta Medica Okayama, 56(4), 171-176.