Improvement of Rice Biomass Yield through QTL-Based Selection

Kazuki Matsubara, Eiji Yamamoto, Nobuya Kobayashi, Takuro Ishii, Junichi Tanaka, Hiroshi Tsunematsu, Satoshi Yoshinaga, Osamu Matsumura, Jun Ichi Yonemaru, Ritsuko Mizobuchi, Toshio Yamamoto, Hiroshi Kato, Masahiro Yano

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Abstract

Biomass yield of rice (Oryza sativa L.) is an important breeding target, yet it is not easy to improve because the trait is complex and phenotyping is laborious. Using progeny derived from a cross between two high-yielding Japanese cultivars, we evaluated whether quantitative trait locus (QTL)-based selection can improve biomass yield. As a measure of biomass yield, we used plant weight (aboveground parts only), which included grain weight and stem and leaf weight. We measured these and related traits in recombinant inbred lines. Phenotypic values for these traits showed a continuous distribution with transgressive segregation, suggesting that selection can affect plant weight in the progeny. Four significant QTLs were mapped for plant weight, three for grain weight, and five for stem and leaf weight (at α = 0.05); some of them overlapped. Multiple regression analysis showed that about 43% of the phenotypic variance of plant weight was significantly explained (P < 0.0001) by six of the QTLs. From F2 plants derived from the same parental cross as the recombinant inbred lines, we divergently selected lines that carried alleles with positive or negative additive effects at these QTLs, and performed successive selfing. In the resulting F6 lines and parents, plant weight significantly differed among the genotypes (at α = 0.05). These results demonstrate that QTL-based selection is effective in improving rice biomass yield.

Original languageEnglish
Pages (from-to)e0151830
JournalPLoS One
Volume11
Issue number3
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Matsubara, K., Yamamoto, E., Kobayashi, N., Ishii, T., Tanaka, J., Tsunematsu, H., Yoshinaga, S., Matsumura, O., Yonemaru, J. I., Mizobuchi, R., Yamamoto, T., Kato, H., & Yano, M. (2016). Improvement of Rice Biomass Yield through QTL-Based Selection. PLoS One, 11(3), e0151830. https://doi.org/10.1371/journal.pone.0151830