Abstract
Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS.
| Original language | English |
|---|---|
| Article number | 0148760 |
| Journal | PLoS One |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2016 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Improved resection and outcome of colon-cancer liver metastasis with fluorescence-guided surgery using in situ GFP labeling with a telomerase-dependent adenovirus in an orthotopic mouse model. / Yano, Shuuya; Takehara, Kiyoto; Miwa, Shinji; Kishimoto, Hiroyuki; Hiroshima, Yukihiko; Murakami, Takashi; Urata, Yasuo; Kagawa, Shunsuke; Bouvet, Michael; Fujiwara, Toshiyoshi; Hoffman, Robert M.
In: PLoS One, Vol. 11, No. 2, 0148760, 01.02.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved resection and outcome of colon-cancer liver metastasis with fluorescence-guided surgery using in situ GFP labeling with a telomerase-dependent adenovirus in an orthotopic mouse model
AU - Yano, Shuuya
AU - Takehara, Kiyoto
AU - Miwa, Shinji
AU - Kishimoto, Hiroyuki
AU - Hiroshima, Yukihiko
AU - Murakami, Takashi
AU - Urata, Yasuo
AU - Kagawa, Shunsuke
AU - Bouvet, Michael
AU - Fujiwara, Toshiyoshi
AU - Hoffman, Robert M.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS.
AB - Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS.
UR - http://www.scopus.com/inward/record.url?scp=84959367745&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84959367745&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0148760
DO - 10.1371/journal.pone.0148760
M3 - Article
C2 - 26849435
AN - SCOPUS:84959367745
VL - 11
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 2
M1 - 0148760
ER -