Abstract
Parkinson's disease is characterized by the accumulation of amyloid, which consists of α-synuclein (α-Syn). To screen compounds with amyloid aggregation inhibitory activity, an effective method for the preparation of α-Syn is a prerequisite. We established a simpler method for α-Syn preparation using freeze-thaw treatment of transformed Escherichia coli. Furthermore, we found that the high-mannose type free N-glycans could prevent α-Syn aggregation.
Original language | English |
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Pages (from-to) | 770-774 |
Number of pages | 5 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 86 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 1 2022 |
Keywords
- free N-glycans
- inhibition of amyloid aggregation
- rapid purification
- α-synuclein
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry