Improved method for preparation and purification of recombinant α-synuclein: high-mannose-type free N-glycan prepared from an edible bean (Vigna angulari, Azuki bean) inhibits α-synuclein aggregation

Shota Kosaka, Makoto Katsube, Megumi Maeda, Yoshinobu Kimura

Research output: Contribution to journalArticlepeer-review

Abstract

Parkinson's disease is characterized by the accumulation of amyloid, which consists of α-synuclein (α-Syn). To screen compounds with amyloid aggregation inhibitory activity, an effective method for the preparation of α-Syn is a prerequisite. We established a simpler method for α-Syn preparation using freeze-thaw treatment of transformed Escherichia coli. Furthermore, we found that the high-mannose type free N-glycans could prevent α-Syn aggregation.

Original languageEnglish
Pages (from-to)770-774
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume86
Issue number6
DOIs
Publication statusPublished - Jun 1 2022

Keywords

  • free N-glycans
  • inhibition of amyloid aggregation
  • rapid purification
  • α-synuclein

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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