Improved high-performance liquid chromatographic detection of paclitaxel in patient's plasma using solid-phase extraction, and semi-micro-bore C18 separation and UV detection

Manabu Suno, Takashi Ono, Shinya Iida, Noriko Umetsu, Ko ichi Ohtaki, Takehiro Yamada, Toshio Awaya, Machiko Satomi, Yoshikazu Tasaki, Keiko Shimizu, Kazuo Matsubara

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Although a number of analytical methods for taxanes have been published, none of them are sufficiently suitable for use in a medical setting. In this study, we established an improved analytical HPLC/UV detection method using a Sep-Pak C18 cartridge for extraction and a semi-micro-bore column for separation. This method employed here reduced chromatographic background signals, and allowed a more sensitive analysis of taxanes in human blood sample. The recovery of taxanes after the solid-phase extraction procedure was over 90%. Chromatographic separation of paclitaxel and docetaxel was achieved within 30 min with no interference peak by a semi-micro-bore column, packed either with C18 (Wakosil 5C18 RS) or pentafluorophenyl (Curosil/Taxol) materials. The method was reproducible with coefficients of variation less than 6%. This analytical procedure was simple and sensitive with lower quantification limit of 3 ng/ml. The improved sensitivity achieved by the popular HPLC/UV apparatus, which is available in hospitals, would vouch safer and more efficient therapy with taxane.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume860
Issue number1
DOIs
Publication statusPublished - Dec 1 2007
Externally publishedYes

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Keywords

  • Docetaxel
  • HPLC/UV
  • Paclitaxel
  • Semi-micro-bore column
  • Sep-Pak C
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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