Improved fertility in gilts and sows after artificial insemination of frozen-thawed boar semen by supplementation of semen extender with caffeine and CaCl2

Shoichiro Yamaguchi, Hiroaki Funahashi, Tetsuya Murakami

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Supplementation of semen extender with caffeine and CaCl2 for artificial insemination (AI) of fresh spermatozoa has been demonstrated to reduce recruitment of uterine polymorphonuclear leukocytes (PMNs) and the activity of phagocytosis. Here, we determined if addition of caffeine and CaCl2 to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cervically inseminated twice with froxzen-thawed boar spermatozoa (25 × 108 cells per dose) suspended in Modena solution (n=7) or modified Beltsville Thawing Solution supplemented with caffeine and CaCl2 (BCC, n=7). The gilts were slaughtered 4 h later, and their oviducts and uterine horns plus the body of the uterus were flushed to recover PMNs and non-phagocytosed spermatozoa. There was no difference in the total number of uterine PMNs between gilts inseminated with Modena solution and those inseminated with BCC (3.8 × 108 vs. 1.5 × 108 cells, respectively); however, the total number of uterine spermatozoa was higher when gilts were inseminated with BCC (40.6 × 106 cells) compared with those inseminated with Modena solution (1.4 × 106 cells, P8 sperm per dose) in Modena (n=21) or BCC (n=21). The overall pregnancy and farrowing rates were higher in females inseminated with BCC (71.4 and 61.9%, respectively) compared with those inseminated with Modena solution (38.1 and 28.6%, respectively, P

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalJournal of Reproduction and Development
Volume55
Issue number6
DOIs
Publication statusPublished - Dec 2009

Fingerprint

semen extenders
caffeine
boars
artificial insemination
gilts
sows
semen
spermatozoa
cells
farrowing rate
dosage
oviducts
phagocytosis
pregnancy rate
thawing
uterus
neutrophils

Keywords

  • Artificial insemination
  • Caffeine and CaCl
  • Frozen-thawed boar semen
  • Polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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title = "Improved fertility in gilts and sows after artificial insemination of frozen-thawed boar semen by supplementation of semen extender with caffeine and CaCl2",
abstract = "Supplementation of semen extender with caffeine and CaCl2 for artificial insemination (AI) of fresh spermatozoa has been demonstrated to reduce recruitment of uterine polymorphonuclear leukocytes (PMNs) and the activity of phagocytosis. Here, we determined if addition of caffeine and CaCl2 to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cervically inseminated twice with froxzen-thawed boar spermatozoa (25 × 108 cells per dose) suspended in Modena solution (n=7) or modified Beltsville Thawing Solution supplemented with caffeine and CaCl2 (BCC, n=7). The gilts were slaughtered 4 h later, and their oviducts and uterine horns plus the body of the uterus were flushed to recover PMNs and non-phagocytosed spermatozoa. There was no difference in the total number of uterine PMNs between gilts inseminated with Modena solution and those inseminated with BCC (3.8 × 108 vs. 1.5 × 108 cells, respectively); however, the total number of uterine spermatozoa was higher when gilts were inseminated with BCC (40.6 × 106 cells) compared with those inseminated with Modena solution (1.4 × 106 cells, P8 sperm per dose) in Modena (n=21) or BCC (n=21). The overall pregnancy and farrowing rates were higher in females inseminated with BCC (71.4 and 61.9{\%}, respectively) compared with those inseminated with Modena solution (38.1 and 28.6{\%}, respectively, P",
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N2 - Supplementation of semen extender with caffeine and CaCl2 for artificial insemination (AI) of fresh spermatozoa has been demonstrated to reduce recruitment of uterine polymorphonuclear leukocytes (PMNs) and the activity of phagocytosis. Here, we determined if addition of caffeine and CaCl2 to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cervically inseminated twice with froxzen-thawed boar spermatozoa (25 × 108 cells per dose) suspended in Modena solution (n=7) or modified Beltsville Thawing Solution supplemented with caffeine and CaCl2 (BCC, n=7). The gilts were slaughtered 4 h later, and their oviducts and uterine horns plus the body of the uterus were flushed to recover PMNs and non-phagocytosed spermatozoa. There was no difference in the total number of uterine PMNs between gilts inseminated with Modena solution and those inseminated with BCC (3.8 × 108 vs. 1.5 × 108 cells, respectively); however, the total number of uterine spermatozoa was higher when gilts were inseminated with BCC (40.6 × 106 cells) compared with those inseminated with Modena solution (1.4 × 106 cells, P8 sperm per dose) in Modena (n=21) or BCC (n=21). The overall pregnancy and farrowing rates were higher in females inseminated with BCC (71.4 and 61.9%, respectively) compared with those inseminated with Modena solution (38.1 and 28.6%, respectively, P

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