Abstract
Recent studies have revealed that bone marrow cells can develop into hepatocytes by in vivo transplantation under certain circumstances. However, little is known about the mechanism of bone marrow cell differentiation into hepatocytes. It is important to determine suitable culture conditions in which bone marrow cells will be differentiated into hepatocytes not only for understanding differentiation mechanisms but also for efficient amplification of hepatocyte-progenitor cells of bone marrow origin, this being a prerequisite for potential therapeutic use. In the present study, we found that hepatocyte growth factor (HGF) receptor (c-Met)- and α-fetoprotein-expressing cells were present in adult rat bone marrow. We also found that these cells also express hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. Using an HGM medium with HGF and EGF, we succeeded in propagating hepatocyte-like cells induced from adult rat bone marrow in culture. These cells were immunocytochemically stained for albumin. By RT-PCR analysis of cultures containing the hepatocyte-like cells, we detected mRNAs of tryptophan-2,3-dioxygenase and tyrosine aminotransferase, markers of hepatocytes at a terminal differentiation stage. The present culture therefore can be a useful resource for cell transplantation therapy for liver diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 24-30 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 298 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Albumin
- Bone marrow cells
- Hepatocytes
- Tryptophan-2,3-dioxygenase
- Tyrosine aminotransferase
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Improved conditions to induce hepatocytes from rat bone marrow cells in culture. / Miyazaki, Masahiro; Akiyama, Ichiro; Sakaguchi, Masakiyo; Nakashima, Emiko; Okada, Mayumi; Kataoka, Ken; Huh, Nam ho.
In: Biochemical and Biophysical Research Communications, Vol. 298, No. 1, 2002, p. 24-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved conditions to induce hepatocytes from rat bone marrow cells in culture
AU - Miyazaki, Masahiro
AU - Akiyama, Ichiro
AU - Sakaguchi, Masakiyo
AU - Nakashima, Emiko
AU - Okada, Mayumi
AU - Kataoka, Ken
AU - Huh, Nam ho
PY - 2002
Y1 - 2002
N2 - Recent studies have revealed that bone marrow cells can develop into hepatocytes by in vivo transplantation under certain circumstances. However, little is known about the mechanism of bone marrow cell differentiation into hepatocytes. It is important to determine suitable culture conditions in which bone marrow cells will be differentiated into hepatocytes not only for understanding differentiation mechanisms but also for efficient amplification of hepatocyte-progenitor cells of bone marrow origin, this being a prerequisite for potential therapeutic use. In the present study, we found that hepatocyte growth factor (HGF) receptor (c-Met)- and α-fetoprotein-expressing cells were present in adult rat bone marrow. We also found that these cells also express hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. Using an HGM medium with HGF and EGF, we succeeded in propagating hepatocyte-like cells induced from adult rat bone marrow in culture. These cells were immunocytochemically stained for albumin. By RT-PCR analysis of cultures containing the hepatocyte-like cells, we detected mRNAs of tryptophan-2,3-dioxygenase and tyrosine aminotransferase, markers of hepatocytes at a terminal differentiation stage. The present culture therefore can be a useful resource for cell transplantation therapy for liver diseases.
AB - Recent studies have revealed that bone marrow cells can develop into hepatocytes by in vivo transplantation under certain circumstances. However, little is known about the mechanism of bone marrow cell differentiation into hepatocytes. It is important to determine suitable culture conditions in which bone marrow cells will be differentiated into hepatocytes not only for understanding differentiation mechanisms but also for efficient amplification of hepatocyte-progenitor cells of bone marrow origin, this being a prerequisite for potential therapeutic use. In the present study, we found that hepatocyte growth factor (HGF) receptor (c-Met)- and α-fetoprotein-expressing cells were present in adult rat bone marrow. We also found that these cells also express hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. Using an HGM medium with HGF and EGF, we succeeded in propagating hepatocyte-like cells induced from adult rat bone marrow in culture. These cells were immunocytochemically stained for albumin. By RT-PCR analysis of cultures containing the hepatocyte-like cells, we detected mRNAs of tryptophan-2,3-dioxygenase and tyrosine aminotransferase, markers of hepatocytes at a terminal differentiation stage. The present culture therefore can be a useful resource for cell transplantation therapy for liver diseases.
KW - Albumin
KW - Bone marrow cells
KW - Hepatocytes
KW - Tryptophan-2,3-dioxygenase
KW - Tyrosine aminotransferase
UR - http://www.scopus.com/inward/record.url?scp=0036401624&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036401624&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(02)02340-9
DO - 10.1016/S0006-291X(02)02340-9
M3 - Article
C2 - 12379214
AN - SCOPUS:0036401624
VL - 298
SP - 24
EP - 30
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -