Improved conditions to induce hepatocytes from rat bone marrow cells in culture

Masahiro Miyazaki, Ichiro Akiyama, Masakiyo Sakaguchi, Emiko Nakashima, Mayumi Okada, Ken Kataoka, Nam ho Huh

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Recent studies have revealed that bone marrow cells can develop into hepatocytes by in vivo transplantation under certain circumstances. However, little is known about the mechanism of bone marrow cell differentiation into hepatocytes. It is important to determine suitable culture conditions in which bone marrow cells will be differentiated into hepatocytes not only for understanding differentiation mechanisms but also for efficient amplification of hepatocyte-progenitor cells of bone marrow origin, this being a prerequisite for potential therapeutic use. In the present study, we found that hepatocyte growth factor (HGF) receptor (c-Met)- and α-fetoprotein-expressing cells were present in adult rat bone marrow. We also found that these cells also express hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. Using an HGM medium with HGF and EGF, we succeeded in propagating hepatocyte-like cells induced from adult rat bone marrow in culture. These cells were immunocytochemically stained for albumin. By RT-PCR analysis of cultures containing the hepatocyte-like cells, we detected mRNAs of tryptophan-2,3-dioxygenase and tyrosine aminotransferase, markers of hepatocytes at a terminal differentiation stage. The present culture therefore can be a useful resource for cell transplantation therapy for liver diseases.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume298
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Cell culture
Bone Marrow Cells
Rats
Hepatocytes
Bone
Cell Culture Techniques
Cells
Bone Marrow
Tryptophan Oxygenase
Proto-Oncogene Proteins c-met
Fetal Proteins
Tyrosine Transaminase
Hepatocyte Growth Factor
Cell Transplantation
Therapeutic Uses
Cell- and Tissue-Based Therapy
Hematopoietic Stem Cells
Stem cells
Epidermal Growth Factor
Tryptophan

Keywords

  • Albumin
  • Bone marrow cells
  • Hepatocytes
  • Tryptophan-2,3-dioxygenase
  • Tyrosine aminotransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Improved conditions to induce hepatocytes from rat bone marrow cells in culture. / Miyazaki, Masahiro; Akiyama, Ichiro; Sakaguchi, Masakiyo; Nakashima, Emiko; Okada, Mayumi; Kataoka, Ken; Huh, Nam ho.

In: Biochemical and Biophysical Research Communications, Vol. 298, No. 1, 2002, p. 24-30.

Research output: Contribution to journalArticle

Miyazaki, Masahiro ; Akiyama, Ichiro ; Sakaguchi, Masakiyo ; Nakashima, Emiko ; Okada, Mayumi ; Kataoka, Ken ; Huh, Nam ho. / Improved conditions to induce hepatocytes from rat bone marrow cells in culture. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 298, No. 1. pp. 24-30.
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