Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate

T. Nitoda, H. Kurumatani, H. Kanzaki, K. Kawazu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.

Original languageEnglish
Pages (from-to)563-565
Number of pages3
JournalPesticide Science
Volume55
Issue number5
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Chitinase inhibitory assay
  • Colloidal chitin
  • Spodoptera litura

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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