Improved bioassay method for plant transformation inhibitors

Hiroshi Kanzaki, Toshihiko Kagemori, Satomi Asano, Kazuyoshi Kawazu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A convenient and quantitative bioassay method for evaluating the efficiency of plant transformation by Agrobacterium tumefaciens is important to search plant transformation inhibitors, possible biochemical probes for study on its mechanism. Our previously reported method, in which the plant transformation had been detected by the expression of β-glucuronidase in transformed plants, was improved. The difference between the previous and the improved methods is the use of suspension-cultured cells of Ageratum conyzoides as the host plant instead of Nicotiana tabacum BY-2; this alteration of the host enabled us to measure the β-glucuronidase activity in plant cells not only fluorometrically but also colorimetrically. The enzyme activity expressed in the cells of A. conyzoides was nearly 100 times higher than that of N. tabacum BY-2, and was enough for detection by colorimetric measurement. The method, therefore, is useful for a convenient determination of inhibitory activity against plant transformation.

Original languageEnglish
Pages (from-to)2328-2333
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number12
DOIs
Publication statusPublished - Jan 1 1998

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Keywords

  • Ageratum conyzoides
  • Agrobacterium tumefaciens
  • Colorimetric measurement
  • Fluorometric measurement
  • GUS assay

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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