Important role of serum proteins associated on the surface of particles in their hepatic disposition

Kentaro Furumoto, Ken-ichi Ogawara, Susumu Nagayama, Yoshinobu Takakura, Mitsuru Hashida, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

To elucidate the important factors for the difference in the hepatic disposition between polystyrene nanospheres with a size of 50 nm (NS-50) and lecithin-coated NS-50 (LNS-50), the liver perfusion studies and the in vitro uptake studies using the cultured Kupffer cells were performed. It was suggested that opsonin-mediated phagocytosis is not significantly involved in the hepatic disposition of LNS-50 in the presence of serum, whereas its involvement in the hepatic uptake of NS-50 was clearly demonstrated. Western blot analysis showed that IgG, complement C3, and fibronectin, well-known opsonins in the serum, adsorbed on the surface of NS-50 in larger amount than on the surface of LNS-50. On the other hand, serum albumin, which was suggested to function as a dysopsonin for the hepatic disposition of NS-50, was associated with both spheres almost to the same extent. These findings suggest that the hepatic disposition of LNS-50 at lower level should be ascribed to the less amount of serum opsonins associated on the surface and that the serum proteins associated with these spheres should be important as a determinant for their hepatic disposition.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalJournal of Controlled Release
Volume83
Issue number1
DOIs
Publication statusPublished - Sep 18 2002

Keywords

  • Hepatic uptake
  • Opsonins
  • Polystyrene nanosphere
  • Receptor-mediated phagocytosis
  • Serum proteins

ASJC Scopus subject areas

  • Pharmaceutical Science

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