Implications of protease M/neurosin in myelination during experimental demyelination and remyelination

Yoshio Bando, Shinji Ito, Yoshiko Nagai, Ryuji Terayama, Mari Kishibe, Ying Ping Jiang, Branka Mitrovic, Takayuki Takahashi, Shigetaka Yoshida

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37 Citations (Scopus)


Protease M/neurosin is a serine protease expressed by oligodendrocytes (OLGs) in the central nervous system (CNS). To investigate the role of protease M/neurosin during experimental demyelination and remyelination, mice were fed cuprizone (bis-cyclohexanon oxaldihydrazone). Semi-quantitative RT-PCR analysis and immunohistochemistry revealed that the expressions of protease M/neurosin mRNA and protein were rapidly reduced in demyelination, whereas the expression of protease M/neurosin was increased in π form of glutathione-S-transferases (GST-π)-positive OLGs during remyelination. Cultured primary OLGs displayed a strong correlation between protease M/neurosin and myelin basic protein (MBP). After tumor necrosis factor-α (TNF-α) and IFN-γ stimulation, these proteins showed colocalization in the oligodendroglial process. The suppression of protease M/neurosin using RNAi reduced the level of MBP mRNA in cultured OLGs. In contrast, the reduced level of protease M/neurosin was not associated with oligodendroglial cell death or differentiation in cultured OLGs. This study identifies that protease M/neurosin in OLGs is closely associated with the expression of the MBP and the PLP gene. Our data emphasize that the maintenance of myelination is an important function of protease M/neurosin in OLGs, suggesting its relation to the oligodendroglial response to myelin disorders.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalNeuroscience Letters
Issue number3
Publication statusPublished - Sep 25 2006


  • Myelination
  • Oligodendrocyte
  • Serine protease

ASJC Scopus subject areas

  • Neuroscience(all)


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