Implication of amphiphysin 1 and dynamin 2 in tubulobulbar complex formation and spermatid release

Norihiro Kusumi, Masami Watanabe, Hiroshi Yamada, Shun Ai Li, Yuji Kashiwakura, Takashi Matsukawa, Atsushi Nagai, Yasutomo Nasu, Hiromi Kumon, Kohji Takei

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Tubulobulbar complexes (TBCs) are composed of several tubular invaginations formed at the plasma membrane of testicular Sertoli cells. TBCs are transiently formed at the contact region with spermatids at spermatogenic stage VII in rat and mouse, and such TBC formation is prerequisite for spermatid release. Since the characteristic structure of TBCs suggests that the molecules implicated in endocytosis could be involved in TBC formation, we here investigated the localization and physiological roles of endocytic proteins, amphiphysin 1 and dynamin 2, at TBCs. We demonstrated by immunofluorescence that the endocytic proteins were concentrated at TBCs, where they colocalized with cytoskeletal proteins, such as actin and vinculin. Immunoelectron microscopy disclosed that both amphiphysin 1 and dynamin 2 were localized on TBC membrane. Next, we histologically examined the testis from amphiphysin 1 deficient {Amph -/-} mice. Morphometric analysis revealed that the number of TBCs was significantly reduced in Amph-/-. The ratio of stage VIII seminiferous tubules was increased, and the ratio of stage IX was conversely decreased in Amph-/-. Moreover, unreleased spermatids in stage VIII seminiferous tubules were increased in Amph-/-, indicating that spermatid release and the following transition from stage VIII to IX was prolonged in Amph-/- mice. These results suggest that amphiphysin 1 and dynamin 2 are involved in TBC formation and spermatid release at Sertoli cells.

Original languageEnglish
Pages (from-to)101-113
Number of pages13
JournalCell Structure and Function
Volume32
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint

Dynamin II
Spermatids
Seminiferous Tubules
Sertoli Cells
Vinculin
Cytoskeletal Proteins
Immunoelectron Microscopy
Endocytosis
Fluorescent Antibody Technique
Testis
Actins
Proteins
Cell Membrane
amphiphysin
Membranes

Keywords

  • Amphiphysin 1
  • Dynamin 2
  • Endocytosis
  • Spermatogenesis
  • Testis
  • Tubulobulbar complex

ASJC Scopus subject areas

  • Physiology
  • Structural Biology
  • Cell Biology

Cite this

Implication of amphiphysin 1 and dynamin 2 in tubulobulbar complex formation and spermatid release. / Kusumi, Norihiro; Watanabe, Masami; Yamada, Hiroshi; Li, Shun Ai; Kashiwakura, Yuji; Matsukawa, Takashi; Nagai, Atsushi; Nasu, Yasutomo; Kumon, Hiromi; Takei, Kohji.

In: Cell Structure and Function, Vol. 32, No. 2, 2007, p. 101-113.

Research output: Contribution to journalArticle

Kusumi, Norihiro ; Watanabe, Masami ; Yamada, Hiroshi ; Li, Shun Ai ; Kashiwakura, Yuji ; Matsukawa, Takashi ; Nagai, Atsushi ; Nasu, Yasutomo ; Kumon, Hiromi ; Takei, Kohji. / Implication of amphiphysin 1 and dynamin 2 in tubulobulbar complex formation and spermatid release. In: Cell Structure and Function. 2007 ; Vol. 32, No. 2. pp. 101-113.
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