Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration

Kentaro Deguchi, Kanji Tsuru, Takeshi Hayashi, Mikiro Takaishi, Mitsuyuki Nagahara, Shoko Nagotani, Yoshihide Sehara, Guang Jin, HanZhe Zhang, Satoshi Hayakawa, Mikio Shoji, Masahiro Miyazaki, Akiyoshi Osaka, Nam Ho Huh, Koji Abe

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

For brain tissue regeneration, any scaffold for migrated or transplanted stem cells with supportive angiogenesis is important once necrotic brain tissue has formed a cavity after injury such as cerebral ischemia. In this study, a new porous gelatin-siloxane hybrid derived from the integration of gelatin and 3-(glycidoxypropyl) trimethoxysilane was implanted as a three-dimensional scaffold into a defect of the cerebral cortex. The porous hybrid implanted into the lesion remained at the same site for 60 days, kept integrity of the brain shape, and attached well to the surrounding brain tissues. Marginal cavities of the scaffolds were occupied by newly formed tissue in the brain, where newly produced vascular endothelial, astroglial, and microglial cells were found with bromodeoxyuridine double positivity, and the numbers of those cells were dose-dependently increased with the addition of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Extension of dendrites was also found from the surrounding cerebral cortex to the newly formed tissue, especially with the addition of bFGF and EGF. The present study showed that a new porous gelatin-siloxane hybrid had biocompatibility after implantation into a lesion of the central nervous system, and thus provided a potential scaffold for cell migration, angiogenesis and dendrite elongation with dose-dependent effects of additive bFGF and EGF.

Original languageEnglish
Pages (from-to)1263-1273
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume26
Issue number10
DOIs
Publication statusPublished - Oct 18 2006

Fingerprint

Siloxanes
Tissue Scaffolds
Gelatin
Regeneration
Fibroblast Growth Factor 2
Epidermal Growth Factor
Brain
Dendrites
Cerebral Cortex
Bromodeoxyuridine
Brain Ischemia
Cell Movement
Blood Vessels
Stem Cells
Central Nervous System
Cell Count
Wounds and Injuries

Keywords

  • Brain tissue defect
  • Porous gelatin-siloxane hybrid
  • Scaffold
  • Tissue regeneration

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration. / Deguchi, Kentaro; Tsuru, Kanji; Hayashi, Takeshi; Takaishi, Mikiro; Nagahara, Mitsuyuki; Nagotani, Shoko; Sehara, Yoshihide; Jin, Guang; Zhang, HanZhe; Hayakawa, Satoshi; Shoji, Mikio; Miyazaki, Masahiro; Osaka, Akiyoshi; Huh, Nam Ho; Abe, Koji.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 26, No. 10, 18.10.2006, p. 1263-1273.

Research output: Contribution to journalArticle

Deguchi, K, Tsuru, K, Hayashi, T, Takaishi, M, Nagahara, M, Nagotani, S, Sehara, Y, Jin, G, Zhang, H, Hayakawa, S, Shoji, M, Miyazaki, M, Osaka, A, Huh, NH & Abe, K 2006, 'Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration', Journal of Cerebral Blood Flow and Metabolism, vol. 26, no. 10, pp. 1263-1273. https://doi.org/10.1038/sj.jcbfm.9600275
Deguchi, Kentaro ; Tsuru, Kanji ; Hayashi, Takeshi ; Takaishi, Mikiro ; Nagahara, Mitsuyuki ; Nagotani, Shoko ; Sehara, Yoshihide ; Jin, Guang ; Zhang, HanZhe ; Hayakawa, Satoshi ; Shoji, Mikio ; Miyazaki, Masahiro ; Osaka, Akiyoshi ; Huh, Nam Ho ; Abe, Koji. / Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration. In: Journal of Cerebral Blood Flow and Metabolism. 2006 ; Vol. 26, No. 10. pp. 1263-1273.
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