Impairment of gingival fibroblast adherence by IL-6/sIL-6R

K. Naruishi, S. Takashiba, F. Nishimura, H. H. Chou, H. Arai, H. Yamada, Y. Murayama

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gp130 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-α5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of β-actin and integrin-α5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.

Original languageEnglish
Pages (from-to)1421-1424
Number of pages4
JournalJournal of dental research
Volume80
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of 'Impairment of gingival fibroblast adherence by IL-6/sIL-6R'. Together they form a unique fingerprint.

  • Cite this

    Naruishi, K., Takashiba, S., Nishimura, F., Chou, H. H., Arai, H., Yamada, H., & Murayama, Y. (2001). Impairment of gingival fibroblast adherence by IL-6/sIL-6R. Journal of dental research, 80(5), 1421-1424. https://doi.org/10.1177/00220345010800050701