Impaired plasma clottability induction through fibrinogen degradation by ASP, a serine protease released from Aeromonas sobria

Takahisa Imamura, Hidetoshi Nitta, Yoshihiro Wada, Hidetomo Kobayashi, Keinosuke Okamoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aeromonas sobria infection often advances to sepsis, in which interaction of bacterial components with plasma proteins possibly causes various disorders. This bacterium releases a serine protease (ASP), a putative virulence factor, and binds to fibrinogen. To study the ASP effect on fibrinogen, we incubated fibrinogen or plasma with ASP and investigated their clotting elicited by thrombin, which converts fibrinogen to a fibrin clot. Enzymatically active ASP retarded plasma clotting in a dose-dependent manner starting at an ASP concentration of 10 nM. ASP also retarded fibrinogen clotting at 3 nM and above, which appeared to correspond to ASP cleavage of fibrinogen at the Aα-chain. Consistent with containing serine protease activity for an ASP-specific substrate, the culture supernatant of an ASP gene-introduced strain retarded plasma and fibrinogen clotting more than that of the wild-type strain. The culture supernatant of an ASP gene-disrupted strain that releases negligible serine protease activity for the ASP-specific substrate did not affect plasma clotting. These results indicate that ASP is the main fibrinogenolytic protease released from A. sobria. Impaired plasma clottability induction through fibrinogen degradation is a new virulence activity of ASP and may contribute to hemorrhagic tendencies in sepsis caused by infection with this bacterium.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalFEMS Microbiology Letters
Volume284
Issue number1
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Aeromonas
Serine Proteases
Fibrinogen
Sepsis
Bacteria
Virulence Factors
Infection
Fibrin

Keywords

  • Aeromonas sobria
  • Bleeding tendency
  • Fibrinogen
  • Plasma clotting
  • Sepsis
  • Serine protease

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Impaired plasma clottability induction through fibrinogen degradation by ASP, a serine protease released from Aeromonas sobria. / Imamura, Takahisa; Nitta, Hidetoshi; Wada, Yoshihiro; Kobayashi, Hidetomo; Okamoto, Keinosuke.

In: FEMS Microbiology Letters, Vol. 284, No. 1, 07.2008, p. 35-42.

Research output: Contribution to journalArticle

Imamura, Takahisa ; Nitta, Hidetoshi ; Wada, Yoshihiro ; Kobayashi, Hidetomo ; Okamoto, Keinosuke. / Impaired plasma clottability induction through fibrinogen degradation by ASP, a serine protease released from Aeromonas sobria. In: FEMS Microbiology Letters. 2008 ; Vol. 284, No. 1. pp. 35-42.
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