Abstract
Background: Gingival overgrowth (GO) is a serious adverse effect associated with the administration of phenytoin (PHT), with PHT-induced GO characterized by a massive accumulation of extracellular matrix components, especially collagen, in gingival connective tissues. However, the etiology of such collagen accumulation is still largely unknown. We examined the effects of PHT on the collagen degradation process leading to collagen accumulation in human gingival fibroblasts (HGF). Methods: HGFs were cultured with various concentrations of PHT and viable cell numbers and collagen amounts were determined. Gene and protein expressions of matrix metalloproteinases (MMP) and tissue inhibitors of MMPs (TIMP) were quantified with reverse transcription-polymerase chain reaction (RT-PCR) analyses and Western blotting, respectively. Cellular endocytosis of collagen was assayed using flow-cytometric analysis. The effects of PHT on extracellular signal-regulated kinase 1/2 (ERK1/2) and inhibitor κB-α (IκB-α) were assayed. Results: The proliferation of HGFs was not affected by PHT, whereas it significantly increased collagen accumulation. Further, the expressions of MMP-1, -2, and -3 were markedly suppressed by PHT, whereas that of TIMP-1 was induced in a dose- and time-dependent manner. PHT also markedly prevented collagen endocytosis by HGFs, which was associated with the suppression of α2β1-integrin expression. In addition, the phosphorylation of ERK1/2 and IκB-α degradation were suppressed by PHT. Conclusions: These results suggest that PHT causes an impaired degradation of collagen by suppression of enzymatic degradation with MMPs/TIMP-1 and α2β1-integrin-mediated endocytosis. Those alterations are likely mediated through the cellular signaling pathways of ERK1/2 and nuclear factor κB. These synergistic effects may cause collagen accumulation, leading to GO.
| Original language | English |
|---|---|
| Pages (from-to) | 941-950 |
| Number of pages | 10 |
| Journal | Journal of Periodontology |
| Volume | 76 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2005 |
| Externally published | Yes |
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Keywords
- Collagen diseases
- Fibroblasts, gingival
- Gingival hyperplasia/etiology
- Metalloproteinases, matrix
- Phenytoin/adverse effects
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Impaired degradation of matrix collagen in human gingival fibroblasts by the antiepileptic drug phenytoin. / Kato, Takahiro; Okahashi, Nobuo; Kawai, Shinji; Kato, Takafumi; Inaba, Hiroaki; Morisaki, Ichijiro; Amano, Atsuo.
In: Journal of Periodontology, Vol. 76, No. 6, 06.2005, p. 941-950.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Impaired degradation of matrix collagen in human gingival fibroblasts by the antiepileptic drug phenytoin
AU - Kato, Takahiro
AU - Okahashi, Nobuo
AU - Kawai, Shinji
AU - Kato, Takafumi
AU - Inaba, Hiroaki
AU - Morisaki, Ichijiro
AU - Amano, Atsuo
PY - 2005/6
Y1 - 2005/6
N2 - Background: Gingival overgrowth (GO) is a serious adverse effect associated with the administration of phenytoin (PHT), with PHT-induced GO characterized by a massive accumulation of extracellular matrix components, especially collagen, in gingival connective tissues. However, the etiology of such collagen accumulation is still largely unknown. We examined the effects of PHT on the collagen degradation process leading to collagen accumulation in human gingival fibroblasts (HGF). Methods: HGFs were cultured with various concentrations of PHT and viable cell numbers and collagen amounts were determined. Gene and protein expressions of matrix metalloproteinases (MMP) and tissue inhibitors of MMPs (TIMP) were quantified with reverse transcription-polymerase chain reaction (RT-PCR) analyses and Western blotting, respectively. Cellular endocytosis of collagen was assayed using flow-cytometric analysis. The effects of PHT on extracellular signal-regulated kinase 1/2 (ERK1/2) and inhibitor κB-α (IκB-α) were assayed. Results: The proliferation of HGFs was not affected by PHT, whereas it significantly increased collagen accumulation. Further, the expressions of MMP-1, -2, and -3 were markedly suppressed by PHT, whereas that of TIMP-1 was induced in a dose- and time-dependent manner. PHT also markedly prevented collagen endocytosis by HGFs, which was associated with the suppression of α2β1-integrin expression. In addition, the phosphorylation of ERK1/2 and IκB-α degradation were suppressed by PHT. Conclusions: These results suggest that PHT causes an impaired degradation of collagen by suppression of enzymatic degradation with MMPs/TIMP-1 and α2β1-integrin-mediated endocytosis. Those alterations are likely mediated through the cellular signaling pathways of ERK1/2 and nuclear factor κB. These synergistic effects may cause collagen accumulation, leading to GO.
AB - Background: Gingival overgrowth (GO) is a serious adverse effect associated with the administration of phenytoin (PHT), with PHT-induced GO characterized by a massive accumulation of extracellular matrix components, especially collagen, in gingival connective tissues. However, the etiology of such collagen accumulation is still largely unknown. We examined the effects of PHT on the collagen degradation process leading to collagen accumulation in human gingival fibroblasts (HGF). Methods: HGFs were cultured with various concentrations of PHT and viable cell numbers and collagen amounts were determined. Gene and protein expressions of matrix metalloproteinases (MMP) and tissue inhibitors of MMPs (TIMP) were quantified with reverse transcription-polymerase chain reaction (RT-PCR) analyses and Western blotting, respectively. Cellular endocytosis of collagen was assayed using flow-cytometric analysis. The effects of PHT on extracellular signal-regulated kinase 1/2 (ERK1/2) and inhibitor κB-α (IκB-α) were assayed. Results: The proliferation of HGFs was not affected by PHT, whereas it significantly increased collagen accumulation. Further, the expressions of MMP-1, -2, and -3 were markedly suppressed by PHT, whereas that of TIMP-1 was induced in a dose- and time-dependent manner. PHT also markedly prevented collagen endocytosis by HGFs, which was associated with the suppression of α2β1-integrin expression. In addition, the phosphorylation of ERK1/2 and IκB-α degradation were suppressed by PHT. Conclusions: These results suggest that PHT causes an impaired degradation of collagen by suppression of enzymatic degradation with MMPs/TIMP-1 and α2β1-integrin-mediated endocytosis. Those alterations are likely mediated through the cellular signaling pathways of ERK1/2 and nuclear factor κB. These synergistic effects may cause collagen accumulation, leading to GO.
KW - Collagen diseases
KW - Fibroblasts, gingival
KW - Gingival hyperplasia/etiology
KW - Metalloproteinases, matrix
KW - Phenytoin/adverse effects
UR - http://www.scopus.com/inward/record.url?scp=21344453286&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21344453286&partnerID=8YFLogxK
U2 - 10.1902/jop.2005.76.6.941
DO - 10.1902/jop.2005.76.6.941
M3 - Article
C2 - 15948689
AN - SCOPUS:21344453286
VL - 76
SP - 941
EP - 950
JO - Journal of Periodontology
JF - Journal of Periodontology
SN - 0022-3492
IS - 6
ER -