Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages

Takayuki Tsukuba, Michiyo Yanagawa, Kuniaki Okamoto, Yoshiko Okamoto, Yoshiyuki Yasuda, Keiichi I. Nakayama, Tomoko Kadowaki, Kenji Yamamoto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE /) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE /macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE /macrophages to MCP-1 and N-formyl-methionyl- leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin β2) and CD 29 (integrin β1) in CatE / macrophages were significantly decreased, thereby reducing cell attachment of CatE / macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE /macrophages.

Original languageEnglish
Pages (from-to)565-573
Number of pages9
JournalJournal of biochemistry
Volume145
Issue number5
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

Keywords

  • Aspartic proteinase
  • Cathepsin E
  • Cell adhesion
  • Chemotaxis
  • Knockout
  • Macrophages

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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