Impaired activation of mast cells upon IgE-mediated antigen stimulation in a stroke-prone spontaneously hypertensive rat strain, SHRSP.Z

Mariko Sakanaka, Kazuyuki Furuta, Atsushi Ichikawa, Satoshi Tanaka

Research output: Contribution to journalArticle

Abstract

We investigated IgE-mediated allergic responses in a metabolic syndrome model rat strain, SHRSP.Z, which develops obesity and hypertension to cast light on the relationship between metabolic disturbances and allergic responses. IgE-mediated cutaneous anaphylactic responses were severely attenuated in this strain regardless of the presence of fa/fa mutation, compared with the parental WKY/Izm strain. Furthermore, in the peritoneal mast cells of both the SHRSP.Z and SHRSP/Izm strains, IgE-mediated activation, such as degranulation and protein tyrosine phosphorylation, was severely impaired whereas no significant differences were found in morphology and number of peritoneal mast cells. Immunoblot analyses revealed that phosphorylation levels of Syk upon IgE-mediated antigen stimulation were significantly decreased and basal expression of linker for activation of T cells (LAT) was down-regulated in peritoneal mast cells of the SHRSP strains. These results suggest that attenuated cutaneous allergic responses in the SHRSP.Z strain might be attributed to impaired FcεRI-mediated signal transduction in mast cells.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalImmunology Letters
Volume128
Issue number1
DOIs
Publication statusPublished - Jan 18 2010
Externally publishedYes

Fingerprint

Inbred SHR Rats
Mast Cells
Immunoglobulin E
Stroke
Antigens
Phosphorylation
Skin
Tyrosine
Signal Transduction
Obesity
Hypertension
T-Lymphocytes
Mutation
Proteins

Keywords

  • Chymase
  • Degranulation
  • Hypertension
  • IgE
  • LAT
  • Mast cell

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Impaired activation of mast cells upon IgE-mediated antigen stimulation in a stroke-prone spontaneously hypertensive rat strain, SHRSP.Z. / Sakanaka, Mariko; Furuta, Kazuyuki; Ichikawa, Atsushi; Tanaka, Satoshi.

In: Immunology Letters, Vol. 128, No. 1, 18.01.2010, p. 74-79.

Research output: Contribution to journalArticle

@article{b03be237ad214744b392395b259ef2f7,
title = "Impaired activation of mast cells upon IgE-mediated antigen stimulation in a stroke-prone spontaneously hypertensive rat strain, SHRSP.Z",
abstract = "We investigated IgE-mediated allergic responses in a metabolic syndrome model rat strain, SHRSP.Z, which develops obesity and hypertension to cast light on the relationship between metabolic disturbances and allergic responses. IgE-mediated cutaneous anaphylactic responses were severely attenuated in this strain regardless of the presence of fa/fa mutation, compared with the parental WKY/Izm strain. Furthermore, in the peritoneal mast cells of both the SHRSP.Z and SHRSP/Izm strains, IgE-mediated activation, such as degranulation and protein tyrosine phosphorylation, was severely impaired whereas no significant differences were found in morphology and number of peritoneal mast cells. Immunoblot analyses revealed that phosphorylation levels of Syk upon IgE-mediated antigen stimulation were significantly decreased and basal expression of linker for activation of T cells (LAT) was down-regulated in peritoneal mast cells of the SHRSP strains. These results suggest that attenuated cutaneous allergic responses in the SHRSP.Z strain might be attributed to impaired FcεRI-mediated signal transduction in mast cells.",
keywords = "Chymase, Degranulation, Hypertension, IgE, LAT, Mast cell",
author = "Mariko Sakanaka and Kazuyuki Furuta and Atsushi Ichikawa and Satoshi Tanaka",
year = "2010",
month = "1",
day = "18",
doi = "10.1016/j.imlet.2009.11.007",
language = "English",
volume = "128",
pages = "74--79",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Impaired activation of mast cells upon IgE-mediated antigen stimulation in a stroke-prone spontaneously hypertensive rat strain, SHRSP.Z

AU - Sakanaka, Mariko

AU - Furuta, Kazuyuki

AU - Ichikawa, Atsushi

AU - Tanaka, Satoshi

PY - 2010/1/18

Y1 - 2010/1/18

N2 - We investigated IgE-mediated allergic responses in a metabolic syndrome model rat strain, SHRSP.Z, which develops obesity and hypertension to cast light on the relationship between metabolic disturbances and allergic responses. IgE-mediated cutaneous anaphylactic responses were severely attenuated in this strain regardless of the presence of fa/fa mutation, compared with the parental WKY/Izm strain. Furthermore, in the peritoneal mast cells of both the SHRSP.Z and SHRSP/Izm strains, IgE-mediated activation, such as degranulation and protein tyrosine phosphorylation, was severely impaired whereas no significant differences were found in morphology and number of peritoneal mast cells. Immunoblot analyses revealed that phosphorylation levels of Syk upon IgE-mediated antigen stimulation were significantly decreased and basal expression of linker for activation of T cells (LAT) was down-regulated in peritoneal mast cells of the SHRSP strains. These results suggest that attenuated cutaneous allergic responses in the SHRSP.Z strain might be attributed to impaired FcεRI-mediated signal transduction in mast cells.

AB - We investigated IgE-mediated allergic responses in a metabolic syndrome model rat strain, SHRSP.Z, which develops obesity and hypertension to cast light on the relationship between metabolic disturbances and allergic responses. IgE-mediated cutaneous anaphylactic responses were severely attenuated in this strain regardless of the presence of fa/fa mutation, compared with the parental WKY/Izm strain. Furthermore, in the peritoneal mast cells of both the SHRSP.Z and SHRSP/Izm strains, IgE-mediated activation, such as degranulation and protein tyrosine phosphorylation, was severely impaired whereas no significant differences were found in morphology and number of peritoneal mast cells. Immunoblot analyses revealed that phosphorylation levels of Syk upon IgE-mediated antigen stimulation were significantly decreased and basal expression of linker for activation of T cells (LAT) was down-regulated in peritoneal mast cells of the SHRSP strains. These results suggest that attenuated cutaneous allergic responses in the SHRSP.Z strain might be attributed to impaired FcεRI-mediated signal transduction in mast cells.

KW - Chymase

KW - Degranulation

KW - Hypertension

KW - IgE

KW - LAT

KW - Mast cell

UR - http://www.scopus.com/inward/record.url?scp=74649084873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74649084873&partnerID=8YFLogxK

U2 - 10.1016/j.imlet.2009.11.007

DO - 10.1016/j.imlet.2009.11.007

M3 - Article

C2 - 19941900

AN - SCOPUS:74649084873

VL - 128

SP - 74

EP - 79

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 1

ER -