Impact of oral commensal bacteria on degradation of periodontal connective tissue in mice

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Manabu Morita, Yoshihiro Shimazaki, Richard P. Darveau

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Innate and adaptive immunosurveillance mechanisms in response to the normal commensal bacteria can affect periodontal innate defense status. However, it is still unclear how commensal bacteria contribute to the inflammatory responses of junctional epithelium (JE) and periodontal connective tissue (PCT). The aim of the present study is to investigate the contribution of commensal bacteria on inflammatory responses in JE and PCT in mice. Methods: The periodontal tissue of germ-free (GF) and specific-pathogen-free (SPF) mice were compared at age 11 to 12 weeks (n = 6 per group). In this study, the number of neutrophils and expression of intercellular adhesion molecule (ICAM)-1, fibroblast growth factor receptor (FGFR)-1, matrix metalloproteinase (MMP)-1, and MMP-8 within the JE and the PCT are evaluated. The collagen density was also determined in PCT stained with picrosirius red (PSR). PSR staining combined with or without polarized light microscopy has been used to assess the organization and maturation of collagen matrix. Results: In the present findings, the area of JE in SPF mice was significantly greater than that in GF mice (P

Original languageEnglish
Pages (from-to)899-905
Number of pages7
JournalJournal of Periodontology
Volume86
Issue number7
DOIs
Publication statusPublished - Jul 1 2015

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Epithelial Attachment
Connective Tissue
Bacteria
Specific Pathogen-Free Organisms
Collagen
Receptor, Fibroblast Growth Factor, Type 1
Matrix Metalloproteinase 8
Polarization Microscopy
Immunologic Monitoring
Matrix Metalloproteinase 1
Intercellular Adhesion Molecule-1
Neutrophils
Staining and Labeling

Keywords

  • Connective tissue
  • Immunity
  • Inflammation
  • Innate
  • Periodontitis

ASJC Scopus subject areas

  • Periodontics

Cite this

Impact of oral commensal bacteria on degradation of periodontal connective tissue in mice. / Irie, Koichiro; Tomofuji, Takaaki; Ekuni, Daisuke; Morita, Manabu; Shimazaki, Yoshihiro; Darveau, Richard P.

In: Journal of Periodontology, Vol. 86, No. 7, 01.07.2015, p. 899-905.

Research output: Contribution to journalArticle

Irie, Koichiro ; Tomofuji, Takaaki ; Ekuni, Daisuke ; Morita, Manabu ; Shimazaki, Yoshihiro ; Darveau, Richard P. / Impact of oral commensal bacteria on degradation of periodontal connective tissue in mice. In: Journal of Periodontology. 2015 ; Vol. 86, No. 7. pp. 899-905.
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