Immunotherapy by a slow delivery system of interleukin-2 in mice models.

Junji Matsuoka, K. Sakagami, Toshiyoshi Fujiwara, T. Onoda, H. Idani, A. Gochi, K. Orita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A sustained release system for interleukin-2 (IL-2), and IL-2 mini-pellet (IL-2 mp), was developed by fusing IL-2 into a needle shaped collagen. Serum concentration of IL-2 after a single subcutaneous injection of the IL-2 mp into C57BL/6 mice remained elevated longer than after an injection of aqueous IL-2. IL-2 in the serum became undetectable by 6h after a subcutaneous injection of 1 x 10(6) unit of IL-2 in phosphate-buffered saline (PBS). In contrast, after a single subcutaneous injection of IL-2 mp containing the same amount of IL-2, the concentration of IL-2 increased to its maximum at 6h after injection, then began to decrease gradually. IL-2 was detected even on the third day after a single subcutaneous injection of one IL-2 mp. Augmentation of NK activity and generation of IL-2 activated killer cells were observed in the spleen from day 1--day 3 after a single subcutaneous injection of IL-2 mp into C57BL/6 mice. This activation was not observed following a single subcutaneous injection of the same amount of IL-2 in PBS. Adoptive immunotherapy by a single subcutaneous injection of IL-2 mp followed by intravenous injections of in vitro cultured IL-2 activated killer cells showed better results in decreasing the number of metastases of Lewis lung carcinoma in C57BL/6 mice than immunotherapy using IL-2 solution.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalActa Medica Okayama
Volume47
Issue number2
Publication statusPublished - Apr 1993

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Immunotherapy
Interleukin-2
Subcutaneous Injections
Inbred C57BL Mouse
Phosphates
Lewis Lung Carcinoma
Adoptive Immunotherapy
Injections
Serum
Intravenous Injections
Needles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Matsuoka, J., Sakagami, K., Fujiwara, T., Onoda, T., Idani, H., Gochi, A., & Orita, K. (1993). Immunotherapy by a slow delivery system of interleukin-2 in mice models. Acta Medica Okayama, 47(2), 79-84.

Immunotherapy by a slow delivery system of interleukin-2 in mice models. / Matsuoka, Junji; Sakagami, K.; Fujiwara, Toshiyoshi; Onoda, T.; Idani, H.; Gochi, A.; Orita, K.

In: Acta Medica Okayama, Vol. 47, No. 2, 04.1993, p. 79-84.

Research output: Contribution to journalArticle

Matsuoka, J, Sakagami, K, Fujiwara, T, Onoda, T, Idani, H, Gochi, A & Orita, K 1993, 'Immunotherapy by a slow delivery system of interleukin-2 in mice models.', Acta Medica Okayama, vol. 47, no. 2, pp. 79-84.
Matsuoka, Junji ; Sakagami, K. ; Fujiwara, Toshiyoshi ; Onoda, T. ; Idani, H. ; Gochi, A. ; Orita, K. / Immunotherapy by a slow delivery system of interleukin-2 in mice models. In: Acta Medica Okayama. 1993 ; Vol. 47, No. 2. pp. 79-84.
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