Immunoscreening of a cDNA library from a lung cancer cell line using autologous patient serum: Identification of XAGE-1b as a dominant antigen and its immunogenicity in lung adenocarcinoma

Ali Mohamed Ali Eldib, Toshiro Ono, Michihide Shimono, Miho Kaneko, Kazuhiko Nakagawa, Ryo Tanaka, Yuji Noguchi, Eiichi Nakayama

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

By serologic identification of antigens by recombinant expression cloning (SEREX) analysis using an autologous lung adenocarcinoma cell line, OU-LU-6, as a cDNA library source, we demonstrated that XAGE-1 was the dominant antigen recognized by serum from a patient. By immunoscreening, we obtained 38 positive cDNA clones consisting of 16 genes designated as OY-LC-1 to -OY-LC-16. OY-LC-1, represented by 18 clones, was identical to XAGE-1. OY-LC-2 to -16, represented by either a single or 2 clones, were identical to known genes shown to be ubiquitously expressed in various normal tissues. RT-PCR analysis showed that of 4 XAGE-1 transcripts-XAGE-1a, b, c and d-XAGE-1b was expressed in OU-LU-6 dominantly. Furthermore, XAGE-1b mRNA was expressed in 4 of 10 lung cancer tissues, whereas no expression was observed in normal tissues. Of 4 XAGE-1b mRNA positive cancer tissues, 3 were adenocarcinoma and one was poorly differentiated squamous cell carcinoma. Of 32 sera from lung cancer patients, 8 sera were reactive with the XAGE-1b product. Those 8 sera were from patients with adenocarcinoma. These findings indicated strong immunogenicity of XAGE-1b in lung adenocarcinoma and suggested its potential use as a target for vaccine-based immunotherapies.

Original languageEnglish
Pages (from-to)558-563
Number of pages6
JournalInternational Journal of Cancer
Volume108
Issue number4
DOIs
Publication statusPublished - Feb 4 2004

Keywords

  • Cancer/testis antigen
  • Lung adenocarcinoma
  • SEREX
  • XAGE-1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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