Immunoreactivity of the JK-132 monoclonal antibody directed against basement membrane collagen in normal and diabetic glomeruli

Hirofumi Makino, Kenichi Shikata, T. Haramoto, K. Hirata, Jun Wada, Z. Ota, T. Hayashi, J. Wieslander, T. Yoshida, K. Yoshioka

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Abstract

The possible involvement of basement membrane-associated collagen (recognized by the monoclonal antibody JK-132) in the evolution of diabetic nephropathy was studied in kidney specimens from seven patients with noninsulin-dependent diabetes mellitus, and its distribution was compared with those of antibodies against α1 to α4 chains of type IV collagen. JK-132, a monoclonal antibody against basement membrane-associated collagen, reacted immunohistochemically exclusively with the mesangial matrix of the glomerular capillary. In contrast, antibodies to the α1 and α2 chains (IV) reacted strongly with mesangial matrix, and less strongly with the glomerular basement membrane (GBM). Antibodies to the α3 and α4 chains (IV) reacted mainly with GBM. In diabetes, JK-132 reacted most extensively with the expanded mesangial matrix, its staining intensity increasing with progression of the diabetic glomerulosclerosis. Antibodies to the α1 and α2 chains (IV) reacted prominently with the expanded mesangial matrix but less strongly with the GBM. Antibodies to the α3 and α4 chains reacted intensely with the thickened GBM. These results suggest that basement membrane-associated collagen differs from α1 to α4 chains of type IV collagen and that basement membrane-associated collagen is a good marker of mesangial expansion in diabetic nephropathy.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalVirchows Archiv
Volume424
Issue number3
DOIs
Publication statusPublished - Apr 1994

Fingerprint

Glomerular Basement Membrane
Basement Membrane
Collagen
Monoclonal Antibodies
Diabetic Nephropathies
Antibodies
Collagen Type IV
Type 2 Diabetes Mellitus
Staining and Labeling
Kidney

Keywords

  • Diabetes mellitus
  • Extracellular matrix
  • Glomerulus
  • Monoclonal antibody
  • Type IV collagen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunoreactivity of the JK-132 monoclonal antibody directed against basement membrane collagen in normal and diabetic glomeruli. / Makino, Hirofumi; Shikata, Kenichi; Haramoto, T.; Hirata, K.; Wada, Jun; Ota, Z.; Hayashi, T.; Wieslander, J.; Yoshida, T.; Yoshioka, K.

In: Virchows Archiv, Vol. 424, No. 3, 04.1994, p. 235-241.

Research output: Contribution to journalArticle

Makino, Hirofumi ; Shikata, Kenichi ; Haramoto, T. ; Hirata, K. ; Wada, Jun ; Ota, Z. ; Hayashi, T. ; Wieslander, J. ; Yoshida, T. ; Yoshioka, K. / Immunoreactivity of the JK-132 monoclonal antibody directed against basement membrane collagen in normal and diabetic glomeruli. In: Virchows Archiv. 1994 ; Vol. 424, No. 3. pp. 235-241.
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