Immunological identification of Vibrio parahaemolyticus: Staphylococcal coagglutination test and enzyme-linked immunosorbent assay

Sumio Shinoda, Mitsuyo Ikeda, Sanae Yamada, Haruaki Kane, Noriko Miyoshi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The species-specific antigenicity of the lateral flagella of Vibrio parahaemolyticus was applied to techniques for immunological identification of the vibrio. One was a coagglutination test using staphylococcal cells sensitized with anti-lateral-flagella antibody bound to protein A located in the staphylococcal cell wall. The other technique was an enzyme-linked immunosorbent assay (ELISA) using a four-layer sandwich procedure. By means of the staphylococcal coagglutination technique, about 106 vibrio cells or 60 ng of flagellar protein could be detected and the whole procedure was performed within 1 h. The sandwich ELISA procedure was more sensitive, but the time required for the whole procedure was about 8 h. Both techniques were specific for V. parahaemolyticus, because no cross-reactivity was observed with other related vibrios, and the tests were not inhibited by heterologous bacterial cells. These results suggest that both techniques are useful for the rapid identification of V. parahaemolyticus in complex samples.

Original languageEnglish
Pages (from-to)301-309
Number of pages9
JournalInternational Journal of Food Microbiology
Volume2
Issue number5
DOIs
Publication statusPublished - 1985

Fingerprint

Vibrio parahaemolyticus
Vibrio
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Flagella
sandwiches
flagellum
testing
Immunologic Techniques
Staphylococcal Protein A
immunologic techniques
cells
methodology
cross reaction
Cell Wall
proteins
cell walls
antibodies
Antibodies
Proteins

Keywords

  • ELISA
  • Immunological identification
  • Staphylococcal coagglutination
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Immunological identification of Vibrio parahaemolyticus : Staphylococcal coagglutination test and enzyme-linked immunosorbent assay. / Shinoda, Sumio; Ikeda, Mitsuyo; Yamada, Sanae; Kane, Haruaki; Miyoshi, Noriko.

In: International Journal of Food Microbiology, Vol. 2, No. 5, 1985, p. 301-309.

Research output: Contribution to journalArticle

Shinoda, Sumio ; Ikeda, Mitsuyo ; Yamada, Sanae ; Kane, Haruaki ; Miyoshi, Noriko. / Immunological identification of Vibrio parahaemolyticus : Staphylococcal coagglutination test and enzyme-linked immunosorbent assay. In: International Journal of Food Microbiology. 1985 ; Vol. 2, No. 5. pp. 301-309.
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