Abstract
It is well established that vesicular nucleotide transporter (VNUT) is responsible for vesicular storage of nucleotides such as ATP, and that VNUT-expressing cells can secrete nucleotides upon exocytosis, playing an important role in purinergic chemical transmission. In the present study, we show that VNUT is expressed in intestinal L cells. Immunohistochemical evidence indicated that VNUT is present in glucagon-like peptide 1 (GLP-1) containing cells in rat intestine. VNUT immunoreactivity is not co-localized with GLP-1, a marker for secretory granules, and synaptophysin, a marker for synaptic-like microvesicles (SLMVs). Essentially the same results were obtained for GLUTag clonal L cells. Sucrose density gradient analysis confirmed that VNUT is present the light fraction, unlike secretory granules. These results demonstrate that intestinal L cells express VNUT in either the unidentified organelles at light density other than secretory granules and SLMVs or a subpopulation of SLMVs, and suggest that L cells are purinergic in nature and secrete nucleotides independent of GLP-1 secretion.
| Original language | English |
|---|---|
| Pages (from-to) | 1090-1095 |
| Number of pages | 6 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 37 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- ATP
- L cell
- Purinergic transmission
- Vesicular nucleotide transporter
ASJC Scopus subject areas
- Pharmaceutical Science
- Pharmacology
- Medicine(all)
Cite this
Immunological identification of vesicular nucleotide transporter in intestinal L cells. / Harada, Yuika; Hiasa, Miki.
In: Biological and Pharmaceutical Bulletin, Vol. 37, No. 7, 2014, p. 1090-1095.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunological identification of vesicular nucleotide transporter in intestinal L cells
AU - Harada, Yuika
AU - Hiasa, Miki
PY - 2014
Y1 - 2014
N2 - It is well established that vesicular nucleotide transporter (VNUT) is responsible for vesicular storage of nucleotides such as ATP, and that VNUT-expressing cells can secrete nucleotides upon exocytosis, playing an important role in purinergic chemical transmission. In the present study, we show that VNUT is expressed in intestinal L cells. Immunohistochemical evidence indicated that VNUT is present in glucagon-like peptide 1 (GLP-1) containing cells in rat intestine. VNUT immunoreactivity is not co-localized with GLP-1, a marker for secretory granules, and synaptophysin, a marker for synaptic-like microvesicles (SLMVs). Essentially the same results were obtained for GLUTag clonal L cells. Sucrose density gradient analysis confirmed that VNUT is present the light fraction, unlike secretory granules. These results demonstrate that intestinal L cells express VNUT in either the unidentified organelles at light density other than secretory granules and SLMVs or a subpopulation of SLMVs, and suggest that L cells are purinergic in nature and secrete nucleotides independent of GLP-1 secretion.
AB - It is well established that vesicular nucleotide transporter (VNUT) is responsible for vesicular storage of nucleotides such as ATP, and that VNUT-expressing cells can secrete nucleotides upon exocytosis, playing an important role in purinergic chemical transmission. In the present study, we show that VNUT is expressed in intestinal L cells. Immunohistochemical evidence indicated that VNUT is present in glucagon-like peptide 1 (GLP-1) containing cells in rat intestine. VNUT immunoreactivity is not co-localized with GLP-1, a marker for secretory granules, and synaptophysin, a marker for synaptic-like microvesicles (SLMVs). Essentially the same results were obtained for GLUTag clonal L cells. Sucrose density gradient analysis confirmed that VNUT is present the light fraction, unlike secretory granules. These results demonstrate that intestinal L cells express VNUT in either the unidentified organelles at light density other than secretory granules and SLMVs or a subpopulation of SLMVs, and suggest that L cells are purinergic in nature and secrete nucleotides independent of GLP-1 secretion.
KW - ATP
KW - L cell
KW - Purinergic transmission
KW - Vesicular nucleotide transporter
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UR - http://www.scopus.com/inward/citedby.url?scp=84904602040&partnerID=8YFLogxK
U2 - 10.1248/bpb.b14-00275
DO - 10.1248/bpb.b14-00275
M3 - Article
C2 - 24989000
AN - SCOPUS:84904602040
VL - 37
SP - 1090
EP - 1095
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 7
ER -