Immunological features and inhibitory effects on enzymatic activity of monoclonal antibodies against Helicobacter pylori urease

Yoshihiro Ikeda, Ryoji Fujii, Keiki Ogino, Keigo Fukushima, Emi Hifumi, Taizo Uda

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The functions of Helicobacter pylori urease, which causes chronic gastritis and gastric ulcers, were investigated in detail at the molecular level. Twenty-six monoclonal antibodies (HpU-1 to HpU-26) were produced against H. pylori urease, which was highly purified in two purification steps. The monoclonal antibodies obtained were characterized with respect to their specificity, immunoaffinity, and inhibitory effect on the enzymatic activity of the urease. The affinity constants of the monoclonal antibodies to the urease were in the range from 1 x 106 to 5 x 109/M. Cross- reactivity experiments with other proteins including Jack bean urease, indicated that these affinities were highly specific to the urease. The antibodies produced could be mainly divided into two categories on the basis of their recognition of the antigen's subunits: one group recognized the α- subunit of the urease and the other recognized the β-subunit. Among the 26 monoclonal antibodies obtained, the antibody designated as HpU-2 showed the strongest inhibitory effect on the enzymatic activity of the urease suppressing the urease activity by 82%. HpU-2 recognized the α-subunit of the urease, but not β-subunit which is considered to include the active site of the urease for binding the nickel ion to hydrolyze urea to ammonia. Its apparent affinity constant was 7.5 x 108/M. The inhibitory effect was enhanced by making two-antibody cocktails of HpU-2 with various other monoclonal antibodies.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalJournal of Fermentation and Bioengineering
Volume86
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Monoclonal antibodies
Urease
Helicobacter pylori
Monoclonal Antibodies
Antibodies
Jacks
Antigens
Urea
Purification
Ammonia
Nickel
Proteins
Ions
Gastritis
Stomach Ulcer
Catalytic Domain
Experiments

Keywords

  • Enzymatic activity
  • Helicobacter pylori urease
  • Inhibitory effect
  • Monoclonal antibody
  • Subunit

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Immunological features and inhibitory effects on enzymatic activity of monoclonal antibodies against Helicobacter pylori urease. / Ikeda, Yoshihiro; Fujii, Ryoji; Ogino, Keiki; Fukushima, Keigo; Hifumi, Emi; Uda, Taizo.

In: Journal of Fermentation and Bioengineering, Vol. 86, No. 3, 1998, p. 271-276.

Research output: Contribution to journalArticle

Ikeda, Yoshihiro ; Fujii, Ryoji ; Ogino, Keiki ; Fukushima, Keigo ; Hifumi, Emi ; Uda, Taizo. / Immunological features and inhibitory effects on enzymatic activity of monoclonal antibodies against Helicobacter pylori urease. In: Journal of Fermentation and Bioengineering. 1998 ; Vol. 86, No. 3. pp. 271-276.
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