The γ-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Sep 15 1993|
ASJC Scopus subject areas
- Molecular Biology