Immunological Detection of F1-ATPase γ-Subunit in Rat Liver Peroxisomes

T. Imanaka, Y. Shiina, Y. Moriyama, S. Ohkuma, T. Takano

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Abstract

The γ-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.

Original languageEnglish
Pages (from-to)1027-1034
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number2
DOIs
Publication statusPublished - Sep 15 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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