Immunological characterization of α antigen of Mycobacterium kansasii

B-cell epitope mapping

M. Naito, Naoya Oohara, S. Matsumoto, T. Yamada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mapping of B-cell epitopes on α antigen of Mycobacterium kansasii (K- α) was carried out by using recombinant truncated K-α fusion peptides. We observed that two immunodominant B-cell epitopes (amino acids 222-268 and 267-306) and one minor epitope (amino acid 249-286) were located in the C- terminal region of K-α. The other three minor B-cell epitopes were mapped in N-terminal (amino acids 80-98 and 99-166) and central (amino acid 174-204) regions of K-α. All defined epitopes were common to Mycobacterium tuberculosis and M. kansasii. Besides these common epitopes, a region in K- α (amino acid 290-319) revealed different reactivities between antibodies against K-α and α antigen of M. tuberculosis. These findings may provide a basis for development of serodiagnosis that can distinguish between M. kansasii and M. tuberculosis infections.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalScandinavian Journal of Immunology
Volume48
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

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Mycobacterium kansasii
B-Lymphocyte Epitopes
Epitope Mapping
Antigens
Amino Acids
Mycobacterium tuberculosis
Epitopes
Immunodominant Epitopes
Mycobacterium Infections
Serologic Tests
Peptides
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Immunological characterization of α antigen of Mycobacterium kansasii : B-cell epitope mapping. / Naito, M.; Oohara, Naoya; Matsumoto, S.; Yamada, T.

In: Scandinavian Journal of Immunology, Vol. 48, No. 1, 1998, p. 73-78.

Research output: Contribution to journalArticle

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