Immunolocalization of keratan sulfate proteoglycan in rat calvaria

H. Nakamura, A. Hirata, Takehito Tsuji, T. Yamamoto

Research output: Contribution to journalArticle

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Abstract

We investigate, by the immunogold method, the localization of keratan sulfate (KS) proteoglycan in rat calvaria in order to clarify the detailed process of intramembranous ossification. KS was localized in bone nodules corresponding to calcified nodules, close to the saggital suture of calvaria. The immunoreactivity decreased in fully calcified regions distant from the suture. Electron microscopic observation revealed that KS was distributed in and around matrix vesicles, among collagen fibrils at the initial crystal deposition stage, and then concentrated in bone nodules. According to the progress of mineralization, KS tended to be localized in the peripheral region of the nodules. In addition, these nodules came in contact with collagen fibrils which also showed KS-positive reactivity. In cell organelles of osteoblasts, KS was detected in the Golgi apparatus. These findings suggest that osteoblasts in intramembranous ossification sites actively synthesize KS. KS in the calcified nodules, as well as other glycosaminoglycans in osteoid, may play an important role in additional and/or collagenous calcification by trapping calcium ions through its negative charge.

Original languageEnglish
Pages (from-to)109-118
Number of pages10
JournalArchives of Histology and Cytology
Volume64
Issue number1
Publication statusPublished - 2001

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Keratan Sulfate
Proteoglycans
Skull
Osteoblasts
Osteogenesis
Sutures
Collagen
Bone and Bones
Golgi Apparatus
Glycosaminoglycans
Organelles
Electrons
Ions
Calcium

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Immunolocalization of keratan sulfate proteoglycan in rat calvaria. / Nakamura, H.; Hirata, A.; Tsuji, Takehito; Yamamoto, T.

In: Archives of Histology and Cytology, Vol. 64, No. 1, 2001, p. 109-118.

Research output: Contribution to journalArticle

Nakamura, H, Hirata, A, Tsuji, T & Yamamoto, T 2001, 'Immunolocalization of keratan sulfate proteoglycan in rat calvaria', Archives of Histology and Cytology, vol. 64, no. 1, pp. 109-118.
Nakamura, H. ; Hirata, A. ; Tsuji, Takehito ; Yamamoto, T. / Immunolocalization of keratan sulfate proteoglycan in rat calvaria. In: Archives of Histology and Cytology. 2001 ; Vol. 64, No. 1. pp. 109-118.
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