IMMUNOLOCALIZATION of INHIBIN/ACTIVIN SUBUNITS and STEROIDOGENIC ENZYMES in the TESTES of AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA)

Qinglin Li; Lu Lu; Qiang Weng; Shig Kawakami; Eriko Saito; Tatsuya Yamamoto; Yuki Yamamoto; Saroch Kaewmanee; Kentaro Nagaoka; Gen Watanabe; Kazuyoshi Taya

doi:10.1638/2015-0091.1

IMMUNOLOCALIZATION of INHIBIN/ACTIVIN SUBUNITS and STEROIDOGENIC ENZYMES in the TESTES of AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA)

Qinglin Li, Lu Lu, Qiang Weng, Shig Kawakami, Eriko Saito, Tatsuya Yamamoto, Yuki Yamamoto, Saroch Kaewmanee, Kentaro Nagaoka, Gen Watanabe, Kazuyoshi Taya

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.

Original language	English
Pages (from-to)	419-422
Number of pages	4
Journal	Journal of Zoo and Wildlife Medicine
Volume	47
Issue number	2
DOIs	https://doi.org/10.1638/2015-0091.1
Publication status	Published - Jun 1 2016

Keywords

African elephant
immunohistochmestry
inhibin
steroidogenic enzyme
testis.

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

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title = "IMMUNOLOCALIZATION of INHIBIN/ACTIVIN SUBUNITS and STEROIDOGENIC ENZYMES in the TESTES of AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA)",

abstract = "In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.",

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author = "Qinglin Li and Lu Lu and Qiang Weng and Shig Kawakami and Eriko Saito and Tatsuya Yamamoto and Yuki Yamamoto and Saroch Kaewmanee and Kentaro Nagaoka and Gen Watanabe and Kazuyoshi Taya",

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AU - Li, Qinglin

AU - Lu, Lu

AU - Weng, Qiang

AU - Kawakami, Shig

AU - Saito, Eriko

AU - Yamamoto, Tatsuya

AU - Yamamoto, Yuki

AU - Kaewmanee, Saroch

AU - Nagaoka, Kentaro

AU - Watanabe, Gen

AU - Taya, Kazuyoshi

PY - 2016/6/1

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N2 - In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.

AB - In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.

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KW - steroidogenic enzyme

KW - testis.

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IMMUNOLOCALIZATION of INHIBIN/ACTIVIN SUBUNITS and STEROIDOGENIC ENZYMES in the TESTES of AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA)

Abstract

Keywords

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