Abstract
In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.
| Original language | English |
|---|---|
| Pages (from-to) | 419-422 |
| Number of pages | 4 |
| Journal | Journal of Zoo and Wildlife Medicine |
| Volume | 47 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 1 2016 |
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Keywords
- African elephant
- immunohistochmestry
- inhibin
- steroidogenic enzyme
- testis.
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
IMMUNOLOCALIZATION of INHIBIN/ACTIVIN SUBUNITS and STEROIDOGENIC ENZYMES in the TESTES of AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA). / Li, Qinglin; Lu, Lu; Weng, Qiang; Kawakami, Shig; Saito, Eriko; Yamamoto, Tatsuya; Yamamoto, Yuki; Kaewmanee, Saroch; Nagaoka, Kentaro; Watanabe, Gen; Taya, Kazuyoshi.
In: Journal of Zoo and Wildlife Medicine, Vol. 47, No. 2, 01.06.2016, p. 419-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - IMMUNOLOCALIZATION of INHIBIN/ACTIVIN SUBUNITS and STEROIDOGENIC ENZYMES in the TESTES of AN ADULT AFRICAN ELEPHANT (LOXODONTA AFRICANA)
AU - Li, Qinglin
AU - Lu, Lu
AU - Weng, Qiang
AU - Kawakami, Shig
AU - Saito, Eriko
AU - Yamamoto, Tatsuya
AU - Yamamoto, Yuki
AU - Kaewmanee, Saroch
AU - Nagaoka, Kentaro
AU - Watanabe, Gen
AU - Taya, Kazuyoshi
PY - 2016/6/1
Y1 - 2016/6/1
N2 - In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.
AB - In this case report, the authors investigated immunolocalization of inhibin α and inhibin/activin βA and βB subunits, as well as steroidogenic enzymes, in the testes of an African elephant. Testes were collected from a reproductively active male African elephant (24 yr old) at autopsy. Histologically, all types of spermatogenic cells including mature-phase spermatozoa were found in the seminiferous tubules. Positive immunostaining for inhibin α and inhibin/activin βA and βB subunits was observed in Sertoli and Leydig cells. In addition, P450scc, 3βHSD, P450c17, and P450arom were also detected in the cytoplasm of Leydig cells. These results suggested that Leydig cells of adult African elephant testes have the ability to synthesize progestin, androgen, and estrogen, whereas both Sertoli and Leydig cells appear as a major source of inhibin secretion in the male African elephant.
KW - African elephant
KW - immunohistochmestry
KW - inhibin
KW - steroidogenic enzyme
KW - testis.
UR - http://www.scopus.com/inward/record.url?scp=84982908192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84982908192&partnerID=8YFLogxK
U2 - 10.1638/2015-0091.1
DO - 10.1638/2015-0091.1
M3 - Article
C2 - 27468011
AN - SCOPUS:84982908192
VL - 47
SP - 419
EP - 422
JO - Journal of Zoo and Wildlife Medicine
JF - Journal of Zoo and Wildlife Medicine
SN - 1042-7260
IS - 2
ER -