Immunolocalization of cell signaling molecules in the granular cell ameloblastoma

Gul San Ara Sathi, Phuu Pwint Han, Ryo Tamamura, Hitoshi Nagatsuka, Hailong Hu, Naoki Katase, Noriyuki Nagai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Bone morphogenic protein (BMP) and Wnt signaling pathway molecules play important roles in cytodifferentiation and cell proliferation. We attempted to localize these signaling molecules in the granular cell ameloblastoma. Materials and methods: Four samples of paraffin-embedded ameloblastoma with granular cells were studied. Immunohistochemistry was performed to detect basement membrane type heparan sulfate (HS) (JM403), cell surface type HS (10E4), heparanase, Wnt-5a, Wnt-2, β-catenin, and BMP-4. Results: In all four samples, strong expression of β-catenin and Wnt-5a was detected within the granular cells, while BMP-4 expression was weak and Wnt-2 was negative. Immunoreactivities of basement membrane type HS, cell surface type HS, and heparanase were variable within granular cells in ameloblastoma. Conclusion: Granular cells in ameloblastoma exhibit abnormal biological behaviors, particularly synthesis and secretion of protein. Synthesis of signaling molecules is upregulated, but secretion is arrested in some cases, while both are lost in other cases.

Original languageEnglish
Pages (from-to)609-614
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume36
Issue number10
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Ameloblastoma
Heparitin Sulfate
Catenins
Basement Membrane
Bone and Bones
Proteins
Wnt Signaling Pathway
Paraffin
Immunohistochemistry
Cell Proliferation

Keywords

  • Ameloblastoma
  • Granular cell
  • Signaling molecules

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Dentistry(all)

Cite this

Immunolocalization of cell signaling molecules in the granular cell ameloblastoma. / Sathi, Gul San Ara; Han, Phuu Pwint; Tamamura, Ryo; Nagatsuka, Hitoshi; Hu, Hailong; Katase, Naoki; Nagai, Noriyuki.

In: Journal of Oral Pathology and Medicine, Vol. 36, No. 10, 11.2007, p. 609-614.

Research output: Contribution to journalArticle

Sathi, Gul San Ara ; Han, Phuu Pwint ; Tamamura, Ryo ; Nagatsuka, Hitoshi ; Hu, Hailong ; Katase, Naoki ; Nagai, Noriyuki. / Immunolocalization of cell signaling molecules in the granular cell ameloblastoma. In: Journal of Oral Pathology and Medicine. 2007 ; Vol. 36, No. 10. pp. 609-614.
@article{564f6ecf9f8f433ab6c9044cecde4bf9,
title = "Immunolocalization of cell signaling molecules in the granular cell ameloblastoma",
abstract = "Background: Bone morphogenic protein (BMP) and Wnt signaling pathway molecules play important roles in cytodifferentiation and cell proliferation. We attempted to localize these signaling molecules in the granular cell ameloblastoma. Materials and methods: Four samples of paraffin-embedded ameloblastoma with granular cells were studied. Immunohistochemistry was performed to detect basement membrane type heparan sulfate (HS) (JM403), cell surface type HS (10E4), heparanase, Wnt-5a, Wnt-2, β-catenin, and BMP-4. Results: In all four samples, strong expression of β-catenin and Wnt-5a was detected within the granular cells, while BMP-4 expression was weak and Wnt-2 was negative. Immunoreactivities of basement membrane type HS, cell surface type HS, and heparanase were variable within granular cells in ameloblastoma. Conclusion: Granular cells in ameloblastoma exhibit abnormal biological behaviors, particularly synthesis and secretion of protein. Synthesis of signaling molecules is upregulated, but secretion is arrested in some cases, while both are lost in other cases.",
keywords = "Ameloblastoma, Granular cell, Signaling molecules",
author = "Sathi, {Gul San Ara} and Han, {Phuu Pwint} and Ryo Tamamura and Hitoshi Nagatsuka and Hailong Hu and Naoki Katase and Noriyuki Nagai",
year = "2007",
month = "11",
doi = "10.1111/j.1600-0714.2007.00580.x",
language = "English",
volume = "36",
pages = "609--614",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - Immunolocalization of cell signaling molecules in the granular cell ameloblastoma

AU - Sathi, Gul San Ara

AU - Han, Phuu Pwint

AU - Tamamura, Ryo

AU - Nagatsuka, Hitoshi

AU - Hu, Hailong

AU - Katase, Naoki

AU - Nagai, Noriyuki

PY - 2007/11

Y1 - 2007/11

N2 - Background: Bone morphogenic protein (BMP) and Wnt signaling pathway molecules play important roles in cytodifferentiation and cell proliferation. We attempted to localize these signaling molecules in the granular cell ameloblastoma. Materials and methods: Four samples of paraffin-embedded ameloblastoma with granular cells were studied. Immunohistochemistry was performed to detect basement membrane type heparan sulfate (HS) (JM403), cell surface type HS (10E4), heparanase, Wnt-5a, Wnt-2, β-catenin, and BMP-4. Results: In all four samples, strong expression of β-catenin and Wnt-5a was detected within the granular cells, while BMP-4 expression was weak and Wnt-2 was negative. Immunoreactivities of basement membrane type HS, cell surface type HS, and heparanase were variable within granular cells in ameloblastoma. Conclusion: Granular cells in ameloblastoma exhibit abnormal biological behaviors, particularly synthesis and secretion of protein. Synthesis of signaling molecules is upregulated, but secretion is arrested in some cases, while both are lost in other cases.

AB - Background: Bone morphogenic protein (BMP) and Wnt signaling pathway molecules play important roles in cytodifferentiation and cell proliferation. We attempted to localize these signaling molecules in the granular cell ameloblastoma. Materials and methods: Four samples of paraffin-embedded ameloblastoma with granular cells were studied. Immunohistochemistry was performed to detect basement membrane type heparan sulfate (HS) (JM403), cell surface type HS (10E4), heparanase, Wnt-5a, Wnt-2, β-catenin, and BMP-4. Results: In all four samples, strong expression of β-catenin and Wnt-5a was detected within the granular cells, while BMP-4 expression was weak and Wnt-2 was negative. Immunoreactivities of basement membrane type HS, cell surface type HS, and heparanase were variable within granular cells in ameloblastoma. Conclusion: Granular cells in ameloblastoma exhibit abnormal biological behaviors, particularly synthesis and secretion of protein. Synthesis of signaling molecules is upregulated, but secretion is arrested in some cases, while both are lost in other cases.

KW - Ameloblastoma

KW - Granular cell

KW - Signaling molecules

UR - http://www.scopus.com/inward/record.url?scp=35348962036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35348962036&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0714.2007.00580.x

DO - 10.1111/j.1600-0714.2007.00580.x

M3 - Article

C2 - 17944754

AN - SCOPUS:35348962036

VL - 36

SP - 609

EP - 614

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 10

ER -