Immunohistochemical Study of Collagen Type IV Alpha Chains and MMP-2, -9 at the Basement Membrane in Oral Carcinogenesis

Ryo Tamamura, Hitoshi Nagatsuka, Mehmet Gunduz, Ichiro Naitoh, Noriyuki Nagai, Lihong Ge, Yoshikazu Sado, Adusa Yabe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane, has six distinct a chains. Matrix metalloproteinases (MMPs) are enzymes that resolve extracellular matrix. Oral squamous cell carcinoma is occurred after precancerous lesions, epithelial dysplasia and carcinoma in situ. We investigated localization of six a chains and MMPs in normal oral mucosal tissue, precancerous lesions, early squamous cell carcinoma immunohistochemically. In normal oral mucosal tissue and epithelial dysplasia, α1 (IV), α2 (IV), α5 (IV) and α 6 (IV) chains were detected continuously along basement membrane. In carcinoma in situ and early squamous cell carcinoma, either α5 (IV) and α6 (IV) chains or all a chains were not stained. In contrast, MMP-2 and MMP-9 that are members of type IV collagenase were stained at the parts of disappearance of a chains. This study suggested that the disappearance of a chains is an important element in carcinogenesis of oral squamous cell carcinoma.

Original languageEnglish
Pages (from-to)237-238
Number of pages2
JournalJournal of Hard Tissue Biology
Volume14
Issue number2
DOIs
Publication statusPublished - 2005

Fingerprint

Collagen Type IV
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Basement Membrane
Collagen
Squamous Cell Carcinoma
Carcinogenesis
Carcinoma in Situ
Matrix Metalloproteinases
Mucous Membrane
Collagenases
Extracellular Matrix
Enzymes
Cells
Epithelial Cells
Metalloproteases
Neoplasms

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Cite this

Immunohistochemical Study of Collagen Type IV Alpha Chains and MMP-2, -9 at the Basement Membrane in Oral Carcinogenesis. / Tamamura, Ryo; Nagatsuka, Hitoshi; Gunduz, Mehmet; Naitoh, Ichiro; Nagai, Noriyuki; Ge, Lihong; Sado, Yoshikazu; Yabe, Adusa.

In: Journal of Hard Tissue Biology, Vol. 14, No. 2, 2005, p. 237-238.

Research output: Contribution to journalArticle

Tamamura, Ryo ; Nagatsuka, Hitoshi ; Gunduz, Mehmet ; Naitoh, Ichiro ; Nagai, Noriyuki ; Ge, Lihong ; Sado, Yoshikazu ; Yabe, Adusa. / Immunohistochemical Study of Collagen Type IV Alpha Chains and MMP-2, -9 at the Basement Membrane in Oral Carcinogenesis. In: Journal of Hard Tissue Biology. 2005 ; Vol. 14, No. 2. pp. 237-238.
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