Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane, has six distinct a chains. Matrix metalloproteinases (MMPs) are enzymes that resolve extracellular matrix. Oral squamous cell carcinoma is occurred after precancerous lesions, epithelial dysplasia and carcinoma in situ. We investigated localization of six a chains and MMPs in normal oral mucosal tissue, precancerous lesions, early squamous cell carcinoma immunohistochemically. In normal oral mucosal tissue and epithelial dysplasia, α1 (IV), α2 (IV), α5 (IV) and α 6 (IV) chains were detected continuously along basement membrane. In carcinoma in situ and early squamous cell carcinoma, either α5 (IV) and α6 (IV) chains or all a chains were not stained. In contrast, MMP-2 and MMP-9 that are members of type IV collagenase were stained at the parts of disappearance of a chains. This study suggested that the disappearance of a chains is an important element in carcinogenesis of oral squamous cell carcinoma.
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Orthopedics and Sports Medicine
- Cell Biology